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Validation of antibody-based recognition by piezoelectric transducers through electroacoustic admittance analysis

Biosensors & bioelectronics, 1998-03, Vol.13 (3), p.259-269 [Peer Reviewed Journal]

1998 ;1998 INIST-CNRS ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 0956-5663 ;EISSN: 1873-4235 ;DOI: 10.1016/S0956-5663(97)00139-5 ;PMID: 9642764

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Title:
Validation of antibody-based recognition by piezoelectric transducers through electroacoustic admittance analysis
Author: Bizet, K. ; Gabrielli, C. ; Perrot, H. ; Therasse, J.
Subjects: Acoustic Impedance Tests ; Adsorption ; Antigen-Antibody Reactions ; Biological and medical sciences ; Biosensing Techniques ; Biosensors ; Biotechnology ; Chemical Sciences ; electroacoustic admittance ; Electrodes ; Electronics, Medical ; enzymes adsorption ; Fundamental and applied biological sciences. Psychology ; Gold ; immunosensors ; Methods. Procedures. Technologies ; Peroxidase - analysis ; Proteins - chemistry ; QCM ; Quartz ; Reproducibility of Results ; Transducers ; Various methods and equipments
Is Part Of: Biosensors & bioelectronics, 1998-03, Vol.13 (3), p.259-269
Description: The development of immunosensors based on piezoelectric transducers is widely investigated due to their attractive potentialities. The quartz crystal microbalance (QCM) may give a direct response signal which characterizes the binding event between a sensitive layer, immobilized onto the surface transducer, and the analyte to be detected. However, for small biomolecules, such as some antigens, it is quite difficult to obtain an observable signal. This is mainly due to the lack of sensitivity of the commonly used QCM (5 to 10 MHz quartz crystal). Moreover, the mass estimated with the QCM response through the Sauerbrey equation and the mass which can be measured thanks to other analytical techniques, in our case an enzymatic assay, are different: the deposited mass is generally overestimated by the QCM. To validate QCM mass measurements and, therefore antigens recognition, the interactions of acoustic shear waves with a biolayer were investigated during enzyme adsorption onto the microbalance gold electrode or during the antibody/antigen binding. Electroacoustic admittance was measured around the resonance frequency of a 27 MHz quartz resonator in parallel with microbalance measurements. The parameters which characterize the quartz microbalance equivalent circuit were compared with the classical microbalance frequency. The mass overestimation, given by the microbalance, could be explained either by modification of the rheological properties of the sensitive layers and/or by an inadequacy of the assay performed.
Publisher: Lausanne: Elsevier B.V
Language: English
Identifier: ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/S0956-5663(97)00139-5
PMID: 9642764
Source: MEDLINE
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Validation of antibody-based recognition by piezoelectric transducers through electroacoustic admittance analysis

1998 ;1998 INIST-CNRS ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 0956-5663 ;EISSN: 1873-4235 ;DOI: 10.1016/S0956-5663(97)00139-5 ;PMID: 9642764

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