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Procedimiento de valoración de un analito en un medio líquido
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Title:
Procedimiento de valoración de un analito en un medio líquido
Author:
BIZET, Karine
;
ROUZEAU, Catherine
;
COMPAIN, Eric
Subjects:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
;
MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS
;
MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS
;
MEASURING
;
PERFORMING OPERATIONS
;
PHYSICS
;
SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
;
SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS
;
TESTING
;
TRANSPORTING
Description:
Procedimiento de valoración de un analito en un medio líquido, que consiste en disponer en este medio partículas magnéticas funcionalizadas mediante ligandos específicos del analito que va a valorarse, en hacer actuar sobre el medio líquido un campo magnético que permite una unión de las partículas magnéticas y la formación de cúmulos de partículas magnéticas, y en determinar la presencia y la concentración del analito a partir de la variación de un parámetro como por ejemplo la densidad óptica del medio líquido, midiéndose el valor de este parámetro antes de aplicar el campo magnético, caracterizado porque comprende una repetición de ciclos que comprenden una primera fase de aplicación del campo magnético al medio líquido y una segunda fase en la que el campo magnético es nulo, midiéndose el parámetro al comienzo y al final de cada ciclo, siendo la duración de aplicación del campo magnético durante cada ciclo inferior a aquella de la reacción entre el analito que va a valorarse y los ligandos de las partículas magnéticas, consistiendo también el procedimiento en calcular a partir de las medidas anteriormente citadas un valor límite de la variación del parámetro para un tiempo infinito de aplicación del campo magnético y en deducir a partir de ello la concentración del analito en el medio líquido. Method of quantifying an analyte in a liquid medium by means of magnetic particles functionalized by specific ligands of the analyte being quantified, consisting in applying a magnetic field to a liquid medium for a time period several times shorter than that of the reaction between the analyte being quantified and the ligands of the magnetic particles, in measuring the optical density of the liquid medium after applying the magnetic field, in repeating this cycle of applying the magnetic field and of measuring the optical density several times over the duration of the reaction between the analyte and the ligands, in calculating, by extrapolation, a limiting value for the optical density for an infinite magnetic field application time and in deducing therefrom the concentration of the analyte in the liquid medium.
Creation Date:
2016
Language:
Spanish
Source:
esp@cenet
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