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Catalase improves saccharification of lignocellulose by reducing lytic polysaccharide monooxygenase-associated enzyme inactivation

Biotechnology letters, 2016-03, Vol.38 (3), p.425-434 [Peer Reviewed Journal]

The Author(s) 2015 ;Springer Science+Business Media Dordrecht 2016 ;ISSN: 0141-5492 ;ISSN: 1573-6776 ;EISSN: 1573-6776 ;DOI: 10.1007/s10529-015-1989-8 ;PMID: 26543036

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Title:
Catalase improves saccharification of lignocellulose by reducing lytic polysaccharide monooxygenase-associated enzyme inactivation
Author: Scott, Brian R ; Huang, Hong Zhi ; Frickman, Jesper ; Halvorsen, Rune ; Johansen, Katja S
Subjects: Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Carbohydrates ; Catalase ; Catalase - metabolism ; Catalysis ; cell walls ; Cellulase ; Cellulases - metabolism ; endo-1,4-beta-glucanase ; enzyme inactivation ; Enzymes ; Fenton chemistry ; fuels ; glucose ; Glucose - metabolism ; half life ; Inactivation ; Kinetic modelling ; Kinetics ; Life Sciences ; Lignin - metabolism ; Lignocellulose ; Lytic polysaccharide monooxygenase ; Mathematical models ; Microbiology ; Mixed Function Oxygenases - metabolism ; Original Research Paper ; Oxidation-Reduction ; oxygen ; Plant Stems - metabolism ; Polysaccharides ; Pretreated wheat straw ; Reactive oxygen species ; Saccharification ; Straw ; t-test ; Triticum - metabolism ; Triticum aestivum ; wheat straw ; wood chips
Is Part Of: Biotechnology letters, 2016-03, Vol.38 (3), p.425-434
Description: OBJECTIVES: Efficient enzymatic saccharification of plant cell wall material is key to industrial processing of agricultural and forestry waste such as straw and wood chips into fuels and chemicals. RESULTS: Saccharification assays were performed on steam-pretreated wheat straw under ambient and O₂-deprived environments and in the absence and presence of a lytic polysaccharide monooxygenase (LPMO) and catalase. A kinetic model was used to calculate catalytic rate and first-order inactivation rate constants of the cellulases from reaction progress curves. The addition of a LPMO significantly (P < 0.01, Student’s T test) enhanced the rate of glucose release from 2.8 to 6.9 h⁻¹ under ambient O₂ conditions. However, this also significantly (P < 0.01, Student’s T test) increased the rate of inactivation of the enzyme mixture, thereby reducing the performance half-life from 65 to 35 h. Decreasing O₂ levels or, strikingly, the addition of catalase significantly reduced (P < 0.01, Student’s T test) enzyme inactivation and, as a consequence, higher efficiency of the cellulolytic enzyme cocktail was achieved. CONCLUSION: Oxidative inactivation of commercial cellulase mixtures is a significant factor influencing the overall saccharification efficiency and the addition of catalase can be used to protect these mixtures from inactivation.
Publisher: Dordrecht: Springer Netherlands
Language: English
Identifier: ISSN: 0141-5492
ISSN: 1573-6776
EISSN: 1573-6776
DOI: 10.1007/s10529-015-1989-8
PMID: 26543036
Source: MEDLINE
Springer Open Access Journals
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ProQuest Central

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Catalase improves saccharification of lignocellulose by reducing lytic polysaccharide monooxygenase-associated enzyme inactivation

The Author(s) 2015 ;Springer Science+Business Media Dordrecht 2016 ;ISSN: 0141-5492 ;ISSN: 1573-6776 ;EISSN: 1573-6776 ;DOI: 10.1007/s10529-015-1989-8 ;PMID: 26543036

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