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The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases

Nature chemical biology, 2016-04, Vol.12 (4), p.298-303 [Peer Reviewed Journal]

Copyright Nature Publishing Group Apr 2016 ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 1552-4450 ;ISSN: 1552-4469 ;EISSN: 1552-4469 ;DOI: 10.1038/nchembio.2029 ;PMID: 26928935

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Title:
The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases
Author: Frandsen, Kristian E H ; Simmons, Thomas J ; Dupree, Paul ; Poulsen, Jens-Christian N ; Hemsworth, Glyn R ; Ciano, Luisa ; Johnston, Esther M ; Tovborg, Morten ; Johansen, Katja S ; von Freiesleben, Pernille ; Marmuse, Laurence ; Fort, Sébastien ; Cottaz, Sylvain ; Driguez, Hugues ; Henrissat, Bernard ; Lenfant, Nicolas ; Tuna, Floriana ; Baldansuren, Amgalanbaatar ; Davies, Gideon J ; Lo Leggio, Leila ; Walton, Paul H
Subjects: Amino Acid Sequence ; Aspergillus oryzae - enzymology ; Aspergillus oryzae - genetics ; Binding Sites ; Biochemistry, Molecular Biology ; Biomass ; Catalytic Domain ; Cellulose - metabolism ; Chitin - metabolism ; Copper ; Copper - metabolism ; Crystallography, X-Ray ; Enzymes ; Fluorescence Resonance Energy Transfer ; Lentinula - enzymology ; Lentinula - genetics ; Life Sciences ; Mixed Function Oxygenases - chemistry ; Mixed Function Oxygenases - genetics ; Mixed Function Oxygenases - metabolism ; Models, Molecular ; Molecular Sequence Data ; Oligosaccharides - chemistry ; Oxidation-Reduction ; Quantitative Methods ; Spectrum analysis ; Structural Biology ; Substrate Specificity
Is Part Of: Nature chemical biology, 2016-04, Vol.12 (4), p.298-303
Description: Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.
Publisher: United States: Nature Publishing Group
Language: English
Identifier: ISSN: 1552-4450
ISSN: 1552-4469
EISSN: 1552-4469
DOI: 10.1038/nchembio.2029
PMID: 26928935
Source: AUTh Library subscriptions: ProQuest Central
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The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases

Copyright Nature Publishing Group Apr 2016 ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 1552-4450 ;ISSN: 1552-4469 ;EISSN: 1552-4469 ;DOI: 10.1038/nchembio.2029 ;PMID: 26928935

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