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Novel IncFII plasmid harbouring blaNDM-4 in a carbapenem-resistant Escherichia coli of pig origin, Italy

Journal of antimicrobial chemotherapy, 2020-12, Vol.75 (12), p.3475-3479 [Peer Reviewed Journal]

The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. 2020 ;The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. ;ISSN: 0305-7453 ;EISSN: 1460-2091 ;DOI: 10.1093/jac/dkaa374 ;PMID: 32835381

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  • Title:
    Novel IncFII plasmid harbouring blaNDM-4 in a carbapenem-resistant Escherichia coli of pig origin, Italy
  • Author: Diaconu, Elena L ; Carfora, Virginia ; Alba, Patricia ; Di Matteo, Paola ; Stravino, Fiorentino ; Buccella, Carmela ; Dell’Aira, Elena ; Onorati, Roberta ; Sorbara, Luigi ; Battisti, Antonio ; Franco, Alessia
  • Subjects: Original Research
  • Is Part Of: Journal of antimicrobial chemotherapy, 2020-12, Vol.75 (12), p.3475-3479
  • Description: Abstract Objectives To the best of our knowledge, we describe the first evidence in Europe of an MDR, blaNDM-4-positive Escherichia coli isolated from a food-producing animal, harboured by a novel IncFII plasmid of which we report the complete sequence. Methods One blaNDM-4-positive E. coli isolated in 2019 from the caecal contents of a fattening pig in Italy was in-depth characterized by combined bioinformatic analysis of Oxford Nanopore long reads and Illumina short reads, for in silico typing, determination of the blaNDM-4 genetic context and full reconstruction of the blaNDM-4–carrying plasmid. Results The isolate belonged to ST641 and to the genoserotype O108:H23 and tested positive for different virulence genes and plasmid replicons. The MDR phenotype of resistance to all β-lactams, carbapenems, sulfamethoxazole and trimethoprim was mediated by blaTEM-1B, blaNDM-4, sul1/sul3 and dfrA12, respectively. The blaNDM-4 gene was harboured by a novel 53 043 bp IncFII plasmid (pMOL412_FII) composed of four main genetic regions, including an MDR region (MRR-NDM-4) of 16 kb carrying blaNDM-4 and several antimicrobial resistance genes located in a class 1 integron. pMOL412_FII was closely related to another ∼90.3 kb plasmid (pM109_FII) harbouring blaNDM-4 in an E. coli isolated from a human patient in Myanmar. Conclusions To the best of our knowledge, we have identified for the first time in Europe an NDM-producing Enterobacterales in livestock and resolved the complete sequence of the novel pMOL412_FII plasmid harbouring blaNDM-4 in an MRR. A global One Health approach, comparing genomic data from different sources and geographical areas, may help to trace back and control possible plasmid-borne carbapenemase gene transmission between animals and humans and along the food chain at international level.
  • Publisher: England: Oxford University Press
  • Language: English
  • Identifier: ISSN: 0305-7453
    EISSN: 1460-2091
    DOI: 10.1093/jac/dkaa374
    PMID: 32835381
  • Source: Oxford Journals Open Access
    Geneva Foundation Free Medical Journals at publisher websites
    Alma/SFX Local Collection
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