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Dicer is required for female reproductive tract development and fertility in the mouse

Molecular reproduction and development, 2009-07, Vol.76 (7), p.678-688 [Peer Reviewed Journal]

Copyright © 2009 Wiley‐Liss, Inc. ;2009 INIST-CNRS ;ISSN: 1040-452X ;EISSN: 1098-2795 ;DOI: 10.1002/mrd.21010 ;PMID: 19197916 ;CODEN: MREDEE

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Title:
Dicer is required for female reproductive tract development and fertility in the mouse
Author: Gonzalez, Gabriel ; Behringer, Richard R.
Subjects: Animals ; Biological and medical sciences ; DEAD-box RNA Helicases - genetics ; DEAD-box RNA Helicases - physiology ; Endoribonucleases - genetics ; Endoribonucleases - physiology ; Estrogens - blood ; Female ; Fertility ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genitalia, Female - embryology ; Genitalia, Female - growth & development ; Genitalia, Female - metabolism ; Genitalia, Female - pathology ; Hormone metabolism and regulation ; Immunohistochemistry ; Infertility, Female - genetics ; Infertility, Female - metabolism ; Inflammation ; Integrases - genetics ; Integrases - metabolism ; Mammalian female genital system ; Mice ; Mice, Transgenic ; Mullerian Ducts - embryology ; Mullerian Ducts - metabolism ; Oviducts - growth & development ; Oviducts - pathology ; Progesterone - blood ; Ribonuclease III ; Vertebrates: reproduction ; Wnt Proteins - genetics ; Wnt Proteins - metabolism
Is Part Of: Molecular reproduction and development, 2009-07, Vol.76 (7), p.678-688
Description: Dicer encodes a riboendonuclease required for microRNA biosynthesis. Dicer was inactivated in Müllerian duct mesenchyme‐derived tissues of the reproductive tract of the mouse, using an Amhr2‐Cre allele. Although Amhr2‐Cre; Dicer conditional mutant males appeared normal and were fertile, mutant females were infertile. In adult mutant females, there was a reduction in the size of the oviducts and uterine horns. The oviducts were less coiled compared to controls and cysts formed at the isthmus near the uterotubal junction. Unfertilized, degenerate oocytes were commonly found within these cysts, indicating a defect in embryo transit. Beads transferred into the mutant oviduct failed to migrate into the uterus. In addition, blastocysts transferred directly into the mutant uterus did not result in pregnancy. Histological analysis demonstrated that the mutant uterus contained less glandular tissue and often the few glands that remained were found within the myometrium, an abnormal condition known as adenomyosis. In adult mutants, there was ectopic expression of Wnt4 and Wnt5a in the luminal epithelium (LE) and glandular epithelium (GE) of the uterus, and Wnt11 was ectopically expressed in GE. These results demonstrate that Dicer is necessary for postnatal differentiation of Müllerian duct mesenchyme‐derived tissues of the female reproductive tract, suggesting that microRNAs are important regulators of female reproductive tract development and fertility. Mol. Reprod. Dev. 76: 678–688, 2009. © 2009 Wiley‐Liss, Inc.
Publisher: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Language: English
Identifier: ISSN: 1040-452X
EISSN: 1098-2795
DOI: 10.1002/mrd.21010
PMID: 19197916
CODEN: MREDEE
Source: MEDLINE
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Dicer is required for female reproductive tract development and fertility in the mouse

Copyright © 2009 Wiley‐Liss, Inc. ;2009 INIST-CNRS ;ISSN: 1040-452X ;EISSN: 1098-2795 ;DOI: 10.1002/mrd.21010 ;PMID: 19197916 ;CODEN: MREDEE

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