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Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses

Proceedings of the National Academy of Sciences - PNAS, 2020-06, Vol.117 (25), p.14503-14511 [Peer Reviewed Journal]

Copyright National Academy of Sciences Jun 23, 2020 ;Distributed under a Creative Commons Attribution 4.0 International License ;2020 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.1922563117 ;PMID: 32513712

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Title:
Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses
Author: Goncalves, Julia ; Bartol, Tomas M. ; Camus, Côme ; Levet, Florian ; Menegolla, Ana Paula ; Sejnowski, Terrence J. ; Sibarita, Jean-Baptiste ; Vivaudou, Michel ; Choquet, Daniel ; Hosy, Eric
Subjects: Activation ; Animals ; Biochemistry, Molecular Biology ; Biological Sciences ; Cells, Cultured ; Electrophysiology ; Embryo, Mammalian ; Female ; Glutamic Acid ; Glutamic Acid - metabolism ; Glutamic acid receptors ; Glutamic acid receptors (metabotropic) ; Hippocampus ; Hippocampus - cytology ; Hippocampus - diagnostic imaging ; Hippocampus - physiology ; Intravital Microscopy ; Life Sciences ; Models, Neurological ; N-Methyl-D-aspartic acid receptors ; Neurons ; Neurons - metabolism ; Neurons - ultrastructure ; Neurotransmitter receptors ; Neurotransmitters ; Patch-Clamp Techniques ; Physiology ; Primary Cell Culture ; Rats ; Rats, Sprague-Dawley ; Receptor, Metabotropic Glutamate 5 ; Receptor, Metabotropic Glutamate 5 - metabolism ; Receptors ; Receptors, AMPA ; Receptors, AMPA - metabolism ; Receptors, N-Methyl-D-Aspartate ; Receptors, N-Methyl-D-Aspartate - metabolism ; Single Molecule Imaging ; Structural Biology ; Synapses ; Synaptic Transmission ; Synaptic Transmission - physiology ; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2020-06, Vol.117 (25), p.14503-14511
Description: The nanoscale co-organization of neurotransmitter receptors facing presynaptic release sites is a fundamental determinant of their coactivation and of synaptic physiology. At excitatory synapses, how endogenous AMPARs, NMDARs, and mGluRs are co-organized inside the synapse and their respective activation during glutamate release are still unclear. Combining single-molecule superresolution microscopy, electrophysiology, and modeling, we determined the average quantity of each glutamate receptor type, their nanoscale organization, and their respective activation. We observed that NMDARs form a unique cluster mainly at the center of the PSD, while AMPARs segregate in clusters surrounding the NMDARs. mGluR5 presents a different organization and is homogenously dispersed at the synaptic surface. From these results, we build a model predicting the synaptic transmission properties of a unitary synapse, allowing better understanding of synaptic physiology.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.1922563117
PMID: 32513712
Source: Geneva Foundation Free Medical Journals at publisher websites
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PubMed Central

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Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses

Copyright National Academy of Sciences Jun 23, 2020 ;Distributed under a Creative Commons Attribution 4.0 International License ;2020 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.1922563117 ;PMID: 32513712

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