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Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response

Cell proliferation, 2021-08, Vol.54 (8), p.e13093-n/a [Peer Reviewed Journal]

2021 The Authors. published by John Wiley & Sons Ltd. ;2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 0960-7722 ;EISSN: 1365-2184 ;DOI: 10.1111/cpr.13093 ;PMID: 34231932

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Title:
Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response
Author: Li, Song ; Luo, Lihua ; He, Yan ; Li, Ruohan ; Xiang, Yangfan ; Xing, Zhenjie ; Li, Yejian ; Albashari, Abdullkhaleg Ali ; Liao, Xiangyan ; Zhang, Keke ; Gao, Liang ; Ye, Qingsong
Subjects: Animals ; Brain ; Carotid arteries ; Cell culture ; Cerebral blood flow ; Cerebral infarction ; cerebral ischaemia‐reperfusion injury ; Cytokines ; Dental materials ; Dental pulp ; dental pulp stem cells ; Deprivation ; Edema ; Ethics ; Exosomes ; HMGB1 protein ; Immunofluorescence ; Impairment ; Infarction ; Inflammation ; Inflammatory response ; Injection ; Injuries ; Injury analysis ; Ischemia ; MyD88 protein ; neuroinflammation ; Neurological complications ; Occlusion ; Original ; oxygen‐glucose deprivation–reperfusion ; Proteins ; Reperfusion ; Stem cells ; Stroke ; Teeth ; TLR4 protein ; Toll-like receptors ; Translocation ; Traumatic brain injury ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Veins & arteries
Is Part Of: Cell proliferation, 2021-08, Vol.54 (8), p.e13093-n/a
Description: Objectives The study aimed to determine whether dental pulp stem cell‐derived exosomes (DPSC‐Exos) exert protective effects against cerebral ischaemia‐reperfusion (I/R) injury and explore its underlying mechanism. Materials and Methods Exosomes were isolated from the culture medium of human DPSC. Adult male C57BL/6 mice were subjected to 2 hours transient middle cerebral artery occlusion (tMCAO) injury followed by 2 hours reperfusion, after which singular injection of DPSC‐Exos via tail vein was administrated. Brain oedema, cerebral infarction and neurological impairment were measured on day 7 after exosomes injection. Then, oxygen‐glucose deprivation–reperfusion (OGD/R) induced BV2 cells were studied to analyse the therapeutic effects of DPSC‐Exos on I/R injury in vitro. Protein levels of TLR4, MyD88, NF‐κB p65, HMGB1, IL‐6, IL‐1β and TNF‐α were determined by western blot or enzyme‐linked immunosorbent assay. The cytoplasmic translocation of HMGB1 was detected by immunofluorescence staining. Results DPSC‐Exos alleviated brain oedema, cerebral infarction and neurological impairment in I/R mice. DPSC‐Exos inhibited the I/R‐mediated expression of TLR4, MyD88 and NF‐κB significantly. DPSC‐Exos also reduced the protein expression of IL‐6, IL‐1β and TNF‐α compared with those of the control both in vitro and in vivo. Meanwhile, DPSC‐Exos markedly decreased the HMGB1 cytoplasmic translocation induced by I/R damage. Conclusions DPSC‐Exos can ameliorate I/R‐induced cerebral injury in mice. Its anti‐inflammatory mechanism might be related with the inhibition of the HMGB1/TLR4/MyD88/NF‐κB pathway. Dental pulp stem cell‐derived exosomes (DPSC‐Exos) alleviated cerebral ischemia‐reperfusion (I/R)‐induced brain oedema, cerebral infarction and neurological impairment in mice. DPSC‐Exos meliorated neuroinflammation after cerebral I/R and oxygen‐ and glucose‐deprived BV2 microglia via the HMGB1/TLR4/MyD88/NF‐κB pathway.
Publisher: Chichester: John Wiley & Sons, Inc
Language: English
Identifier: ISSN: 0960-7722
EISSN: 1365-2184
DOI: 10.1111/cpr.13093
PMID: 34231932
Source: Journals@Ovid Open Access Journal Collection Rolling
PubMed Central
ProQuest Central
DOAJ Directory of Open Access Journals

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Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response

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