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Understanding the role of immunoaffinity-based mass spectrometry methods for clinical applications

Clinical chemistry (Baltimore, Md.), 2012-12, Vol.58 (12), p.1620-1622 [Peer Reviewed Journal]

COPYRIGHT 2012 American Association for Clinical Chemistry, Inc. ;Copyright American Association for Clinical Chemistry Dec 2012 ;ISSN: 0009-9147 ;EISSN: 1530-8561 ;DOI: 10.1373/clinchem.2012.193714 ;PMID: 23082004

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  • Title:
    Understanding the role of immunoaffinity-based mass spectrometry methods for clinical applications
  • Author: Ackermann, Bradley L
  • Subjects: Antibodies - immunology ; Binding sites (Biochemistry) ; Chromatography ; Humans ; Hydroxycholecalciferols - immunology ; Mass spectrometry ; Medical research ; Medicine, Experimental ; Methods ; Proteins
  • Is Part Of: Clinical chemistry (Baltimore, Md.), 2012-12, Vol.58 (12), p.1620-1622
  • Description: Because of differing affinities of the capture antibody for the various metabolites and their wide range in concentrations (1000-fold), Laha et al. performed a detailed binding assessment by LCMS/ MS using synthetic standards to verify the suitability of their approach. [...]because 2knowninterfering metabolites were shown not to bind to the antibody, the authors were able to use a shorter chromatographic run time. SISCAPA is particularly useful for analyzing large protein mixtures and has been extensively applied to the verification of disease-related biomarkers (10, 11 ). Because both methods incorporate stable isotope-labeled internal standards (SIL-IS) for the target peptide, good analytical precision is obtained. In these situations, rapid development of fit-for-purpose assays, often for limited-analyte mixtures, can determine which proteins are essential to measure in clinical studies by use of more highly validated methods. Because sample throughput is not critical at this stage, IA-MS methods are quite practical to implement.
  • Publisher: England: American Association for Clinical Chemistry, Inc
  • Language: English
  • Identifier: ISSN: 0009-9147
    EISSN: 1530-8561
    DOI: 10.1373/clinchem.2012.193714
    PMID: 23082004
  • Source: AUTh Library subscriptions: ProQuest Central
    MEDLINE
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