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Pathology of congenital Zika syndrome in Brazil: a case series

The Lancet (British edition), 2016-08, Vol.388 (10047), p.898-904 [Peer Reviewed Journal]

Elsevier Ltd ;2016 Elsevier Ltd ;Copyright © 2016 Elsevier Ltd. All rights reserved. ;Copyright Elsevier Limited Aug 27, 2016 ;ISSN: 0140-6736 ;EISSN: 1474-547X ;DOI: 10.1016/S0140-6736(16)30883-2 ;PMID: 27372395 ;CODEN: LANCAO

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Title:
Pathology of congenital Zika syndrome in Brazil: a case series
Author: Martines, Roosecelis Brasil, MD ; Bhatnagar, Julu, PhD ; de Oliveira Ramos, Ana Maria, Prof ; Davi, Helaine Pompeia Freire, MD ; Iglezias, Silvia D'Andretta, MD ; Kanamura, Cristina Takami, MSc ; Keating, M Kelly, DVM ; Hale, Gillian, MD ; Silva-Flannery, Luciana, PhD ; Muehlenbachs, Atis, MD ; Ritter, Jana, DVM ; Gary, Joy, DVM ; Rollin, Dominique, MD ; Goldsmith, Cynthia S, MGS ; Reagan-Steiner, Sarah, MD ; Ermias, Yokabed, MPH ; Suzuki, Tadaki, MD ; Luz, Kleber G, Prof ; de Oliveira, Wanderson Kleber, MSc ; Lanciotti, Robert, PhD ; Lambert, Amy, PhD ; Shieh, Wun-Ju, MD ; Zaki, Sherif R, Dr
Subjects: Abortion, Spontaneous - virology ; Adult ; Aedes ; Antigens, Viral - isolation & purification ; Autopsy ; Brain - pathology ; Brain - virology ; Brazil ; Congenital diseases ; Fatal Outcome ; Female ; Flaviviridae ; Health risk assessment ; Humans ; Immunohistochemistry - methods ; Infant ; Internal Medicine ; Limb Deformities, Congenital - diagnostic imaging ; Limb Deformities, Congenital - virology ; Male ; Maternal & child health ; Microcephaly - pathology ; Microcephaly - virology ; Neuroglia - pathology ; Neuroglia - virology ; Newborn babies ; Pathology ; Placenta - pathology ; Placenta - virology ; Pregnancy ; Pregnancy Complications, Infectious - pathology ; Pregnancy Complications, Infectious - virology ; Pregnancy Trimester, First ; Pregnancy Trimester, Second ; Pregnancy Trimester, Third ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - isolation & purification ; Syndrome ; Ultrasonography, Prenatal ; Zika virus ; Zika Virus - immunology ; Zika Virus - isolation & purification ; Zika Virus Infection - congenital ; Zika Virus Infection - pathology
Is Part Of: The Lancet (British edition), 2016-08, Vol.388 (10047), p.898-904
Description: Summary Background Zika virus is an arthropod-borne virus that is a member of the family Flaviviridae transmitted mainly by mosquitoes of the genus Aedes . Although usually asymptomatic, infection can result in a mild and self-limiting illness characterised by fever, rash, arthralgia, and conjunctivitis. An increase in the number of children born with microcephaly was noted in 2015 in regions of Brazil with high transmission of Zika virus. More recently, evidence has been accumulating supporting a link between Zika virus and microcephaly. Here, we describe findings from three fatal cases and two spontaneous abortions associated with Zika virus infection. Methods In this case series, formalin-fixed paraffin-embedded tissue samples from five cases, including two newborn babies with microcephaly and severe arthrogryposis who died shortly after birth, one 2-month-old baby, and two placentas from spontaneous abortions, from Brazil were submitted to the Infectious Diseases Pathology Branch at the US Centers for Disease Control and Prevention (Atlanta, GA, USA) between December, 2015, and March, 2016. Specimens were assessed by histopathological examination, immunohistochemical assays using a mouse anti-Zika virus antibody, and RT-PCR assays targeting the NS5 and envelope genes. Amplicons of RT-PCR positive cases were sequenced for characterisation of strains. Findings Viral antigens were localised to glial cells and neurons and associated with microcalcifications in all three fatal cases with microcephaly. Antigens were also seen in chorionic villi of one of the first trimester placentas. Tissues from all five cases were positive for Zika virus RNA by RT-PCR, and sequence analyses showed highest identities with Zika virus strains isolated from Brazil during 2015. Interpretation These findings provide strong evidence of a link between Zika virus infection and different congenital central nervous system malformations, including microcephaly as well as arthrogryposis and spontaneous abortions. Funding None.
Publisher: England: Elsevier Ltd
Language: English
Identifier: ISSN: 0140-6736
EISSN: 1474-547X
DOI: 10.1016/S0140-6736(16)30883-2
PMID: 27372395
CODEN: LANCAO
Source: ProQuest One Psychology
MEDLINE
ProQuest Central

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Pathology of congenital Zika syndrome in Brazil: a case series

Elsevier Ltd ;2016 Elsevier Ltd ;Copyright © 2016 Elsevier Ltd. All rights reserved. ;Copyright Elsevier Limited Aug 27, 2016 ;ISSN: 0140-6736 ;EISSN: 1474-547X ;DOI: 10.1016/S0140-6736(16)30883-2 ;PMID: 27372395 ;CODEN: LANCAO

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