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SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods

Revista do Instituto de Medicina Tropical de São Paulo, 2020-01, Vol.62, p.e44-8 [Peer Reviewed Journal]

2020. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. ;ISSN: 1678-9946 ;ISSN: 0036-4665 ;EISSN: 1678-9946 ;DOI: 10.1590/S1678-9946202062044 ;PMID: 32609256

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Title:
SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods
Author: Oliveira, Beatriz Araujo ; Oliveira, Lea Campos de ; Sabino, Ester Cerdeira ; Okay, Thelma Suely
Subjects: 2019-nCoV ; Asymptomatic ; Betacoronavirus - genetics ; Betacoronavirus - isolation & purification ; Coronavirus Infections - diagnosis ; Coronavirus Infections - epidemiology ; Coronaviruses ; COVID-19 ; Cytokines ; Disease transmission ; Enzyme-Linked Immunosorbent Assay - methods ; Genomes ; Humans ; Immunoassay - methods ; Immunochromatography ; Immunoenzyme Techniques - methods ; Infections ; Influenza ; Laboratories ; Nucleic Acid Amplification Techniques - methods ; Nucleic Acid Amplification Techniques - standards ; Pandemics ; Patients ; Pneumonia, Viral - diagnosis ; Pneumonia, Viral - epidemiology ; Polymerase chain reaction ; Protein Array Analysis - methods ; Proteins ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Review ; RNA polymerase ; RT-PCR ; SARS-CoV-2 ; Serological methods ; Severe acute respiratory syndrome coronavirus 2 ; Symptom Assessment - methods ; TROPICAL MEDICINE ; Tumor necrosis factor-TNF ; Viruses
Is Part Of: Revista do Instituto de Medicina Tropical de São Paulo, 2020-01, Vol.62, p.e44-8
Description: Coronavirus disease 2019 (COVID-19) is an infectious disease initially reported in China and currently worldwide dispersed caused by a new coronavirus (SARS-CoV-2 or 2019-nCoV) affecting more than seven million people around the world causing more than 400 thousand deaths (on June 8th, 2020). The diagnosis of COVID-19 is based on the clinical and epidemiological history of the patient. However, the gold standard for COVID-19 diagnosis is the viral detection through the amplification of nucleic acids. Although the quantitative Reverse-Transcription Polymerase Chain Reaction (RT-PCR) has been described as the gold standard for diagnosing COVID-19, there are several difficulties involving its use. Here we comment on RT-PCR and describe alternative tests developed for the diagnosis of COVID-19.
Publisher: Brazil: Instituto de Medicina Tropical de Sao Paulo
Language: English;Portuguese
Identifier: ISSN: 1678-9946
ISSN: 0036-4665
EISSN: 1678-9946
DOI: 10.1590/S1678-9946202062044
PMID: 32609256
Source: SciELO
MEDLINE
PubMed Central
Coronavirus Research Database
ProQuest Central
DOAJ Directory of Open Access Journals

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SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods

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