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GAD1 expression and its methylation as indicators of malignant behavior in thymic epithelial tumors

Oncology letters, 2021-06, Vol.21 (6), p.483-483, Article 483

Copyright: © Soejima et al. ;COPYRIGHT 2021 Spandidos Publications ;Copyright Spandidos Publications UK Ltd. 2021 ;Copyright: © Soejima et al. 2021 ;ISSN: 1792-1074 ;EISSN: 1792-1082 ;DOI: 10.3892/ol.2021.12744 ;PMID: 33968199

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Title:
GAD1 expression and its methylation as indicators of malignant behavior in thymic epithelial tumors
Author: Soejima, Shiho ; Kondo, Kazuya ; Tsuboi, Mitsuhiro ; Muguruma, Kyoka ; Tegshee, Bilguun ; Kawakami, Yukikiyo ; Kajiura, Koichiro ; Kawakita, Naoya ; Toba, Hiroaki ; Yoshida, Mitsuteru ; Takizawa, Hiromitsu ; Tangoku, Akira
Subjects: Antibodies ; Binding sites ; Cancer ; Chemotherapy ; Comparative analysis ; DNA methylation ; Epigenetic inheritance ; Epigenetics ; Epithelial tumors ; GABA ; Gene expression ; Genes ; Genetic transcription ; Genomes ; Genomics ; Glutamate ; Glutamate decarboxylase ; Immunohistochemistry ; Kinases ; Medical prognosis ; Methylation ; Mutation ; Myasthenia gravis ; Oncology ; Prognosis ; RNA ; Stains & staining ; Sulfites ; Surgery ; Thymus gland ; Tumors
Is Part Of: Oncology letters, 2021-06, Vol.21 (6), p.483-483, Article 483
Description: Thymic epithelial tumors (TETs) comprise thymomas and thymic carcinoma (TC). TC has more aggressive features and a poorer prognosis than thymomas. Genetic and epigenetic alterations in thymomas and TC have been investigated in an attempt to identify novel target molecules for TC. In the present study, genome-wide screening was performed on aberrantly methylated CpG islands in thymomas and TC, and the glutamate decarboxylase 1 gene ( ) was identified as the 4th significantly hypermethylated CpG island in TC compared with thymomas. catalyzes the production of γ-aminobutyric acid from L-glutamic acid. expression is abundant in the brain but rare in other tissues, including the thymus. A total of 73 thymomas and 17 TC tissues were obtained from 90 patients who underwent surgery or biopsy at Tokushima University Hospital between 1990 and 2017. DNA methylation was examined by bisulfite pyrosequencing, and the mRNA and protein expression levels of were analyzed using reverse transcription-quantitative PCR and immunohistochemistry, respectively. The DNA methylation levels of were significantly higher in TC tissues than in the normal thymus and thymoma tissues, and methylation exhibited high sensitivity and specificity for discriminating between TC and thymoma. The mRNA and protein expression levels of were significantly higher in TC tissues than in thymomas. Patients with TET with high DNA hypermethylation and high mRNA and protein expression levels had significantly shorter relapse-free survival rates than those with low levels. In conclusion, significantly more epigenetic alterations were observed in TC tissues compared with in thymomas, which may contribute to the clinical features and prognosis of patients.
Publisher: Greece: Spandidos Publications
Language: English
Identifier: ISSN: 1792-1074
EISSN: 1792-1082
DOI: 10.3892/ol.2021.12744
PMID: 33968199
Source: PubMed Central
ProQuest Central

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GAD1 expression and its methylation as indicators of malignant behavior in thymic epithelial tumors

Copyright: © Soejima et al. ;COPYRIGHT 2021 Spandidos Publications ;Copyright Spandidos Publications UK Ltd. 2021 ;Copyright: © Soejima et al. 2021 ;ISSN: 1792-1074 ;EISSN: 1792-1082 ;DOI: 10.3892/ol.2021.12744 ;PMID: 33968199

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