Language:

Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli

Polish journal of veterinary sciences, 2020-01, Vol.23 (1), p.143-152 [Peer Reviewed Journal]

Copyright© by the Polish Academy of Sciences. ;Copyright Polish Academy of Sciences, Committee of Veterinary Sciences 2020 ;ISSN: 1505-1773 ;EISSN: 2300-2557 ;DOI: 10.24425/pjvs.2020.132758 ;PMID: 32233297

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Author: Zhao, L Y ; Niu, J H ; Gao, X L ; Liu, C N ; Liu, S M ; Jiang, N ; Lv, X P ; Zheng, S M
Subjects: Antibiotics ; Antigen presentation ; Antigens, Bacterial - genetics ; Antigens, Bacterial - metabolism ; Deoxyribonucleic acid ; Design ; Developing countries ; Diarrhea ; DNA ; DNA polymerase ; DNA, Bacterial - genetics ; Drug resistance ; E coli ; Enterotoxigenic Escherichia coli - genetics ; Enterotoxigenic Escherichia coli - metabolism ; Escherichia coli ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; Fimbriae Proteins - genetics ; Fimbriae Proteins - metabolism ; Gene amplification ; Genes ; Hogs ; LDCs ; Methods ; Molecular Diagnostic Techniques - methods ; Nucleic Acid Amplification Techniques - methods ; Pathogens ; Pili ; Polymerase chain reaction ; Sensitivity and Specificity ; Thermal cycling ; Weaning
Is Part Of: Polish journal of veterinary sciences, 2020-01, Vol.23 (1), p.143-152
Description: Enterotoxigenic Escherichia coli (ETEC) is the causative agent of a wide range of diseases, which are the important cause of illness and mortality in piglets. ETEC strains expressing F4 fimbriae are frequently associated with post-weaning diarrhea (PWD) and lead to great economic losses in swine production industry worldwide. The aim of this study was to establish a rapid and effective isothermal amplification method for detection of F4 fimbriae. Loop-mediated isothermal amplification (LAMP), Polymerase spiral reaction (PSR) and cross-priming ampli- fication (CPA) were used to develop and optimize the detection method first time. Subsequently, the specificity and sensitivity of these methods were evaluated, and the clinical samples were detected with these methods. All the F4-positive samples could produce ladder-like amplifica- tions products and lead the chromogenic substrate SYBR Green I produce green fluorescence, while in blank control and negative samples lack of this pattern or remained orange. The sensi- tivity of LAMP and CPA were 10 times higher than PSR method. Meanwhile, these three methods were validated with clinical samples, 7 were found positive, while 125 samples were negative, the testing results were consisted with the real-time PCR method. These findings suggested that the isothermal amplification based on the F4 fimbriae is a rapid, effective and sensitive method under resource constrains.
Publisher: Germany: Polish Academy of Sciences, Committee of Veterinary Sciences
Language: English;Polish
Identifier: ISSN: 1505-1773
EISSN: 2300-2557
DOI: 10.24425/pjvs.2020.132758
PMID: 32233297
Source: MEDLINE
ProQuest Central

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli

Copyright© by the Polish Academy of Sciences. ;Copyright Polish Academy of Sciences, Committee of Veterinary Sciences 2020 ;ISSN: 1505-1773 ;EISSN: 2300-2557 ;DOI: 10.24425/pjvs.2020.132758 ;PMID: 32233297

Searching Remote Databases, Please Wait