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OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice

Plant molecular biology, 2017-11, Vol.95 (4-5), p.345-357 [Peer Reviewed Journal]

Springer Science+Business Media B.V. 2017 ;Plant Molecular Biology is a copyright of Springer, (2017). All Rights Reserved. ;ISSN: 0167-4412 ;EISSN: 1573-5028 ;DOI: 10.1007/s11103-017-0654-0 ;PMID: 28856519

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Title:
OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice
Author: Tang, Jianpeng ; Zhang, Wenwei ; Wen, Kai ; Chen, Gaoming ; Sun, Juan ; Tian, Yunlu ; Tang, Weijie ; Yu, Jun ; An, Hongzhou ; Wu, Tingting ; Kong, Fei ; Terzaghi, William ; Wang, Chunming ; Wan, Jianmin
Subjects: Biochemistry ; Biomedical and Life Sciences ; Biosynthesis ; Chloroplasts ; Chloroplasts - genetics ; Chloroplasts - physiology ; Chloroplasts - ultrastructure ; Cloning ; Complementation ; DNA-directed RNA polymerase ; Editing ; Genetic Complementation Test ; Life Sciences ; Membranes ; Mutants ; Mutation ; Organelle Biogenesis ; Oryza ; Oryza - genetics ; Oryza - physiology ; Oryza - ultrastructure ; Peptides ; Plant Leaves - genetics ; Plant Leaves - physiology ; Plant Leaves - ultrastructure ; Plant Pathology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; Plastids ; Proteins ; Regulatory mechanisms (biology) ; Ribonucleic acid ; Rice ; RNA ; RNA Editing ; RNA Splicing ; RNA, Chloroplast - genetics ; RNA, Messenger - genetics ; Seedlings ; Seedlings - genetics ; Seedlings - physiology ; Seedlings - ultrastructure ; Splicing ; Thylakoid membranes ; Thylakoids - genetics ; Thylakoids - physiology ; Thylakoids - ultrastructure ; Transcription
Is Part Of: Plant molecular biology, 2017-11, Vol.95 (4-5), p.345-357
Description: Key message OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice. The chloroplast has its own genetic material and genetic system, but it is also regulated by nuclear-encoded genes. However, little is known about nuclear-plastid regulatory mechanisms underlying early chloroplast biogenesis in rice. In this study, we isolated and characterized a mutant, osppr6 , that showed early chloroplast developmental defects leading to albino leaves and seedling death. We found that the osppr6 mutant failed to form thylakoid membranes. Using map-based cloning and complementation tests, we determined that OsPPR6 encoded a new Pentatricopeptide Repeat (PPR) protein localized in plastids. In the osppr6 mutants, mRNA levels of plastidic genes transcribed by the plastid-encoded RNA polymerase decreased, while those of genes transcribed by the nuclear-encoded RNA polymerase increased. Western blot analyses validated these expression results. We further investigated plastidic RNA editing and splicing in the osppr6 mutants and found that the ndhB transcript was mis-edited and the ycf3 transcript was mis-spliced. Therefore, we demonstrate that OsPPR6, a PPR protein, regulates early chloroplast biogenesis and participates in editing of ndhB and splicing of ycf3 transcripts in rice.
Publisher: Dordrecht: Springer Netherlands
Language: English
Identifier: ISSN: 0167-4412
EISSN: 1573-5028
DOI: 10.1007/s11103-017-0654-0
PMID: 28856519
Source: MEDLINE
ProQuest Central

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OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice

Springer Science+Business Media B.V. 2017 ;Plant Molecular Biology is a copyright of Springer, (2017). All Rights Reserved. ;ISSN: 0167-4412 ;EISSN: 1573-5028 ;DOI: 10.1007/s11103-017-0654-0 ;PMID: 28856519

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