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Role of non-specific interactions in the phase-separation and maturation of macromolecules

PLoS computational biology, 2022-05, Vol.18 (5), p.e1010067-e1010067 [Peer Reviewed Journal]

COPYRIGHT 2022 Public Library of Science ;2022 Krishnan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2022 Krishnan et al 2022 Krishnan et al ;ISSN: 1553-7358 ;ISSN: 1553-734X ;EISSN: 1553-7358 ;DOI: 10.1371/journal.pcbi.1010067 ;PMID: 35533203

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  • Title:
    Role of non-specific interactions in the phase-separation and maturation of macromolecules
  • Author: Krishnan, Rakesh ; Ranganathan, Srivastav ; Maji, Samir K ; Padinhateeri, Ranjith
  • Schlessinger, Avner
  • Subjects: Biology and Life Sciences ; Biomolecules ; Cell division ; Chains (polymeric) ; Entropy ; Macromolecules ; Phase separation ; Physical Sciences ; Physiological aspects ; Polymers ; Polymers - chemistry ; Proteins ; Questions ; Self assembly ; Separation (Technology) ; Simulation
  • Is Part Of: PLoS computational biology, 2022-05, Vol.18 (5), p.e1010067-e1010067
  • Description: Phase separation of biomolecules could be mediated by both specific and non-specific interactions. How the interplay between non-specific and specific interactions along with polymer entropy influences phase separation is an open question. We address this question by simulating self-associating molecules as polymer chains with a short core stretch that forms the specifically interacting functional interface and longer non-core regions that participate in non-specific/promiscuous interactions. Our results show that the interplay of specific (strength, ϵsp) and non-specific interactions (strength, ϵns) could result in phase separation of polymers and its transition to solid-like aggregates (mature state). In the absence of ϵns, the polymer chains do not dwell long enough in the vicinity of each other to undergo phase separation and transition into a mature state. On the other hand, in the limit of strong ϵns, the assemblies cannot transition into the mature state and form a non-specific assembly, suggesting an optimal range of interactions favoring mature multimers. In the scenario where only a fraction (Nfrac) of the non-core regions participate in attractive interactions, we find that slight modifications to either ϵns or Nfrac can result in dramatically altered self-assembled states. Using a combination of heterogeneous and homogeneous mix of polymers, we establish how this interplay between interaction energies dictates the propensity of biomolecules to find the correct binding partner at dilute concentrations in crowded environments.
  • Publisher: United States: Public Library of Science
  • Language: English
  • Identifier: ISSN: 1553-7358
    ISSN: 1553-734X
    EISSN: 1553-7358
    DOI: 10.1371/journal.pcbi.1010067
    PMID: 35533203
  • Source: Open Access: PubMed Central
    Geneva Foundation Free Medical Journals at publisher websites
    MEDLINE
    Public Library of Science
    ProQuest Central
    DOAJ Directory of Open Access Journals
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