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Hypermethylation of RASSF1A in Human and Rhesus Placentas

The American journal of pathology, 2007-03, Vol.170 (3), p.941-950 [Peer Reviewed Journal]

American Society for Investigative Pathology ;2007 American Society for Investigative Pathology ;2007 INIST-CNRS ;Copyright © American Society for Investigative Pathology 2007 ;ISSN: 0002-9440 ;EISSN: 1525-2191 ;DOI: 10.2353/ajpath.2007.060641 ;PMID: 17322379 ;CODEN: AJPAA4

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Title:
Hypermethylation of RASSF1A in Human and Rhesus Placentas
Author: Chiu, Rossa W.K ; Chim, Stephen S.C ; Wong, Ivy H.N ; Wong, Cesar S.C ; Lee, Wing-Shan ; To, Ka F ; Tong, Joanna H.M ; Yuen, Ryan K.C ; Shum, Alisa S.W ; Chan, John K.C ; Chan, Lisa Y.S ; Yuen, Jessie W.F ; Tong, Yu K ; Weier, Jingly F ; Ferlatte, Christy ; Leung, Tse N ; Lau, Tze K ; Lo, Kwok W ; Lo, Y.M. Dennis
Subjects: Amino Acid Sequence ; Animals ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - genetics ; Cell Line, Tumor ; DNA Methylation ; DNA Primers ; Epigenesis, Genetic ; Female ; Gene Expression ; Genes, Tumor Suppressor ; Humans ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Lasers ; Macaca mulatta ; Medical sciences ; Mice ; Microdissection ; Molecular Sequence Data ; Pathology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Placenta - physiology ; Pregnancy ; Primates ; Promoter Regions, Genetic ; Regular ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Tumor Suppressor Proteins - genetics
Is Part Of: The American journal of pathology, 2007-03, Vol.170 (3), p.941-950
Description: The pseudomalignant nature of the placenta prompted us to search for tumor suppressor gene hypermethylation, a phenomenon widely reported in cancer, in the human placenta. Nine tumor suppressor genes were studied. Hypermethylation of the Ras association domain family 1 A ( RASSF1A ) gene was found in human placentas from all three trimesters of pregnancy but was absent in other fetal tissues. Hypermethylation of Rassf1 was similarly observed in placentas from the rhesus monkey but not the mouse. An inverse relationship between RASSF1A promoter methylation and gene expression was demonstrated by bisulfite sequencing of microdissected placental cells and immunohistochemical staining of placental tissue sections using an anti-RASSF1A antibody. Treatment of choriocarcinoma cell lines, JAR and JEG3, by 5-aza-2′-deoxycytidine and trichostatin A led to reduction in RASSF1A methylation but increased expression. These observations extend the analogy between the primate placenta and malignant tumors to the epigenetic level.
Publisher: Bethesda, MD: Elsevier Inc
Language: English
Identifier: ISSN: 0002-9440
EISSN: 1525-2191
DOI: 10.2353/ajpath.2007.060641
PMID: 17322379
CODEN: AJPAA4
Source: Geneva Foundation Free Medical Journals at publisher websites
MEDLINE
PubMed Central

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Hypermethylation of RASSF1A in Human and Rhesus Placentas

American Society for Investigative Pathology ;2007 American Society for Investigative Pathology ;2007 INIST-CNRS ;Copyright © American Society for Investigative Pathology 2007 ;ISSN: 0002-9440 ;EISSN: 1525-2191 ;DOI: 10.2353/ajpath.2007.060641 ;PMID: 17322379 ;CODEN: AJPAA4

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