Language:

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles

Nature cell biology, 2020-02, Vol.22 (2), p.187-199 [Peer Reviewed Journal]

COPYRIGHT 2020 Nature Publishing Group ;COPYRIGHT 2020 Nature Publishing Group ;2020© The Author(s), under exclusive licence to Springer Nature Limited 2020 ;ISSN: 1465-7392 ;EISSN: 1476-4679 ;DOI: 10.1038/s41556-019-0450-y ;PMID: 31932738

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles
Author: Leidal, Andrew M ; Huang, Hector H ; Marsh, Timothy ; Solvik, Tina ; Zhang, Dachuan ; Ye, Jordan ; Kai, FuiBoon ; Goldsmith, Juliet ; Liu, Jennifer Y ; Huang, Yu-Hsin ; Monkkonen, Teresa ; Vlahakis, Ariadne ; Huang, Eric J ; Goodarzi, Hani ; Yu, Li ; Wiita, Arun P ; Debnath, Jayanta
Subjects: Adaptor Proteins, Vesicular Transport - deficiency ; Adaptor Proteins, Vesicular Transport - genetics ; Animals ; Autophagosomes - chemistry ; Autophagosomes - metabolism ; Autophagy ; Autophagy - genetics ; Autophagy-Related Protein 7 - deficiency ; Autophagy-Related Protein 7 - genetics ; Autophagy-Related Proteins - deficiency ; Autophagy-Related Proteins - genetics ; Binding ; Binding proteins ; Biological Transport ; Biotinylation ; Cargo ; Conjugation ; Extracellular Vesicles - chemistry ; Extracellular Vesicles - metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; HEK293 Cells ; Heterogeneous-Nuclear Ribonucleoprotein K - genetics ; Heterogeneous-Nuclear Ribonucleoprotein K - metabolism ; Humans ; Intracellular Signaling Peptides and Proteins - genetics ; Intracellular Signaling Peptides and Proteins - metabolism ; Lysosomes - chemistry ; Lysosomes - metabolism ; Machinery ; Matrix Attachment Region Binding Proteins - genetics ; Matrix Attachment Region Binding Proteins - metabolism ; Mice ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - metabolism ; Non-coding RNA ; Nuclear Matrix-Associated Proteins - genetics ; Nuclear Matrix-Associated Proteins - metabolism ; Packaging ; Phagocytosis ; Protein binding ; Proteins ; Proteomics ; Proteomics - methods ; RAW 264.7 Cells ; Receptors, Estrogen - genetics ; Receptors, Estrogen - metabolism ; Ribonucleic acid ; Ribonucleoprotein K ; RNA ; RNA, Small Untranslated - genetics ; RNA, Small Untranslated - metabolism ; RNA-binding protein ; RNA-Binding Proteins - classification ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; Secretion ; Secretome ; Sphingomyelin phosphodiesterase ; Sphingomyelin Phosphodiesterase - genetics ; Sphingomyelin Phosphodiesterase - metabolism ; Target recognition ; Vesicles
Is Part Of: Nature cell biology, 2020-02, Vol.22 (2), p.187-199
Description: Traditionally viewed as an autodigestive pathway, autophagy also facilitates cellular secretion; however, the mechanisms underlying these processes remain unclear. Here, we demonstrate that components of the autophagy machinery specify secretion within extracellular vesicles (EVs). Using a proximity-dependent biotinylation proteomics strategy, we identify 200 putative targets of LC3-dependent secretion. This secretome consists of a highly interconnected network enriched in RNA-binding proteins (RBPs) and EV cargoes. Proteomic and RNA profiling of EVs identifies diverse RBPs and small non-coding RNAs requiring the LC3-conjugation machinery for packaging and secretion. Focusing on two RBPs, heterogeneous nuclear ribonucleoprotein K (HNRNPK) and scaffold-attachment factor B (SAFB), we demonstrate that these proteins interact with LC3 and are secreted within EVs enriched with lipidated LC3. Furthermore, their secretion requires the LC3-conjugation machinery, neutral sphingomyelinase 2 (nSMase2) and LC3-dependent recruitment of factor associated with nSMase2 activity (FAN). Hence, the LC3-conjugation pathway controls EV cargo loading and secretion.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 1465-7392
EISSN: 1476-4679
DOI: 10.1038/s41556-019-0450-y
PMID: 31932738
Source: MEDLINE
ProQuest Central

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles

COPYRIGHT 2020 Nature Publishing Group ;COPYRIGHT 2020 Nature Publishing Group ;2020© The Author(s), under exclusive licence to Springer Nature Limited 2020 ;ISSN: 1465-7392 ;EISSN: 1476-4679 ;DOI: 10.1038/s41556-019-0450-y ;PMID: 31932738

Searching Remote Databases, Please Wait