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PSX-9 Gonadectomy Is Not Correlated with Altered T-Lymphocyte Populations in Senior Labrador Retrievers

Journal of animal science, 2023-11, Vol.101 (Supplement_3), p.509-510 [Peer Reviewed Journal]

The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. 2023 ;The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. ;ISSN: 0021-8812 ;EISSN: 1525-3163 ;DOI: 10.1093/jas/skad281.601

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  • Title:
    PSX-9 Gonadectomy Is Not Correlated with Altered T-Lymphocyte Populations in Senior Labrador Retrievers
  • Author: Mccracken, Fiona ; Fowler, Jason ; Dickerson, Sarah M ; Timlin, Claire ; Skaggs, Patrick ; Coon, Craig N
  • Subjects: Adaptive systems ; Animal population ; Animal populations ; Autoimmune diseases ; Blood ; Body weight ; CD3 antigen ; CD4 antigen ; CD8 antigen ; Flow cytometry ; Gonadectomy ; Immune system ; Lasers ; Lymphocytes ; Lymphocytes T ; Peripheral blood ; Populations ; Rivers
  • Is Part Of: Journal of animal science, 2023-11, Vol.101 (Supplement_3), p.509-510
  • Description: Abstract Retrospective studies have suggested that gonadectomies, such as the spays and neuters common in companion animal populations, may be correlated with the development of autoimmune conditions later in the life of an animale. This study investigated the potential influences of gonadectomy on peripheral blood lymphocyte populations through flow cytometry. Twenty senior Labrador Retrievers (10 males and 10 females) were randomly selected from Four Rivers Kennel’s existing research colony according to their intact or altered status. Groups were balanced by age and body weight (average 11.4 y/o and 64.5 kg) as both have been previously shown to alter lymphocyte populations. A single blood collection was performed and stained with canine cross-reactive conjugated antibodies CD3-FITC, CD4-Superbright 600, CD8-Superbright 702, and a fixable lime viability stain (Invitrogen, Waltham MA). Samples were processed through a whole blood based lyse no-wash method and analyzed on an Attune NXT Flow Cytometer equipped with blue and violet lasers (ThermoFisher Waltham, MA). Measurement of cell populations was completed on a defined volume of each sample, and all subsets were gated to live, singlet lymphocytes. Statistical significance was determined through one-way ANOVAs on JMP Pro 16 (Cary, NC). There were no significant differences in CD4+/CD8+ ratio between groups (P = 0.56), or total lymphocyte populations (P = 0.34). Additionally, there were no significant differences between groups regarding CD3+ MFI (P = 0.25), CD8+ MFI (P = 0.70), or CD4+ MFI (P = 0.43). Overall, in age and body weight balanced senior Labrador Retrievers, spay or neuter status did not significantly influence T-cell populations. This suggests that previous gonadectomy does not influence the adaptive immune system as an animal ages. Further research is needed to clarify the relationship between gonadectomy and the later development of autoimmune disorders.
  • Publisher: US: Oxford University Press
  • Language: English
  • Identifier: ISSN: 0021-8812
    EISSN: 1525-3163
    DOI: 10.1093/jas/skad281.601
  • Source: PubMed Central
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