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Bacterial cellulose matrices to develop enzymatically active paper

(c) Springer Verlag, 2020 info:eu-repo/semantics/openAccess ;ISSN: 0969-0239 ;EISSN: 1572-882X ;DOI: 10.1007/s10570-020-03025-9

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  • Title:
    Bacterial cellulose matrices to develop enzymatically active paper
  • Author: Buruaga Ramiro, Carolina ; Valls Vidal, Cristina ; Valenzuela Mayorga, Susana Valeria ; Roncero, M. Blanca ; Pastor Blasco, Francisco I. Javier ; Díaz Lucea, Pilar ; Martínez Martínez, Josefina
  • Subjects: Bacteria ; Bacteris ; Cellulose ; Cel·lulosa
  • Description: This work studies the suitability of bacterial cellulose (BC) matrices to prepare enzymatically active nanocomposites, in a framework of more environmentally friendly methodologies. After BC production and purification, two kind of matrices were obtained: BC in aqueous suspension and BC paper. A lipase was immobilised onto the BC matrices by physical adsorption, obtaining Lipase/BC nanocomposites. Neither morphology nor crystallinity, measured by scanning electron microscopy (SEM) and X-Ray diffractometry (XRD) respectively, of the BC were affected by the binding of the protein. The activity of Lipase/BC suspension and Lipase/BC paper was tested under different conditions, and the operational properties of the enzyme were evaluated. A shift towards higher temperatures, a broader pH activity range, and slight differences in the substrate preference were observed in the immobilised lipase, compared with the free enzyme. Specific activity was higher for Lipase/BC suspension (4.2 U/mg) than for Lipase/BC paper (1.7 U/mg) nanocomposites. However, Lipase/BC paper nanocomposites showed improved thermal stability, reusability, and durability. Enzyme immobilised onto BC paper retained 60% of its activity after 48 h at 60 ºC. It maintained 100% of the original activity after being recycled 10 times at pH 7 at 60 ºC and it remained active after being stored for more than a month at room temperature. The results suggested that lipase/BC nanocomposites are promising biomaterials for the development of green biotechnological devices with potential application in industrials bioprocesses of detergents and food industry and biomedicine. Lipase/BC paper nanocomposite might be a key component of bioactive paper for developing simple, handheld, and disposable devices.
  • Publisher: Springer Verlag
  • Creation Date: 2020-01
  • Language: English
  • Identifier: ISSN: 0969-0239
    EISSN: 1572-882X
    DOI: 10.1007/s10570-020-03025-9
  • Source: Diposit Digital de la Universitat de Barcelona
    ProQuest Central
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