Language:

A Low-Cost Microfluidic-Based Detection Device for Rapid Identification and Quantification of Biomarkers-Based on a Smartphone

Biosensors (Basel), 2023-07, Vol.13 (7), p.753 [Peer Reviewed Journal]

COPYRIGHT 2023 MDPI AG ;2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2023 by the authors. 2023 ;ISSN: 2079-6374 ;EISSN: 2079-6374 ;DOI: 10.3390/bios13070753 ;PMID: 37504151

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
A Low-Cost Microfluidic-Based Detection Device for Rapid Identification and Quantification of Biomarkers-Based on a Smartphone
Author: Yang, Chonghui ; Yang, Yujing ; Zhao, Gaozhen ; Wang, Huan ; Dai, Yang ; Huang, Xiaowen
Subjects: Antibodies ; Antigens ; Biochemical analysis ; Biological markers ; Biological models (mathematics) ; Biomarkers ; Biotechnology ; Diagnosis ; Fluorescence ; glass capillary ; Image analysis ; Image processing ; Immunoassay ; Light ; Low cost ; Medical diagnosis ; Medical research ; Microfluidics ; Monoclonal antibodies ; Proteins ; RGB ; Sandwich structures ; Severe acute respiratory syndrome coronavirus 2 ; Smart phones ; smartphone ; Smartphones
Is Part Of: Biosensors (Basel), 2023-07, Vol.13 (7), p.753
Description: The sensitive and rapid detection of microsamples is crucial for early diagnosis of diseases. The short response times and low sample volume requirements of microfluidic chips have shown great potential in early diagnosis, but there are still shortcomings such as complex preparation processes and high costs. We developed a low-cost smartphone-based fluorescence detection device (Smartphone-BFDD) without precision equipment for rapid identification and quantification of biomarkers on glass capillary. The device combines microfluidic technology with RGB image analysis, effectively reducing the sample volume to 20 μL and detection time to only 30 min. For the sensitivity of the device, we constructed a standard sandwich immunoassay (antibody-antigen-antibody) in a glass capillary using the N-protein of SARS-CoV-2 as a biological model, realizing a low limit of detection (LOD, 40 ng mL ). This device provides potential applications for different biomarkers and offers wide use for rapid biochemical analysis in biomedical research.
Publisher: Switzerland: MDPI AG
Language: English
Identifier: ISSN: 2079-6374
EISSN: 2079-6374
DOI: 10.3390/bios13070753
PMID: 37504151
Source: PubMed (Medline)
Coronavirus Research Database
ROAD: Directory of Open Access Scholarly Resources
ProQuest Central
DOAJ Directory of Open Access Journals

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

A Low-Cost Microfluidic-Based Detection Device for Rapid Identification and Quantification of Biomarkers-Based on a Smartphone

Searching Remote Databases, Please Wait