skip to main content
Guest
My Research
My Account
Sign out
Sign in
This feature requires javascript
Library Search
Find Databases
Browse Search
E-Journals A-Z
E-Books A-Z
Citation Linker
Help
Language:
English
Vietnamese
This feature required javascript
This feature requires javascript
Primo Search
All Library Resources
All
Course Materials
Course Materials
Search For:
Clear Search Box
Search in:
All Library Resources
Or hit Enter to replace search target
Or select another collection:
Search in:
All Library Resources
Search in:
Print Resources
Search in:
Digital Resources
Search in:
Online E-Resources
Advanced Search
Browse Search
This feature requires javascript
Search Limited to:
Search Limited to:
Resource type
criteria input
All items
Books
Articles
Images
Audio Visual
Maps
Graduate theses
Show Results with:
criteria input
that contain my query words
with my exact phrase
starts with
Show Results with:
Search type Index
criteria input
anywhere in the record
in the title
as author/creator
in subject
Full Text
ISBN
ISSN
TOC
Keyword
Field
Show Results with:
in the title
Show Results with:
anywhere in the record
in the title
as author/creator
in subject
Full Text
ISBN
ISSN
TOC
Keyword
Field
This feature requires javascript
Frequent methylation of RASSF1 and RARB in urine sediments from patients with early stage prostate cancer
Medicina (Kaunas, Lithuania), 2011-01, Vol.47 (3), p.147-153
[Peer Reviewed Journal]
ISSN: 1648-9144 ;EISSN: 1648-9144 ;DOI: 10.3390/medicina47030020 ;PMID: 21822036
Full text available
Citations
Cited by
View Online
Details
Recommendations
Reviews
Times Cited
External Links
This feature requires javascript
Actions
Add to My Research
Remove from My Research
E-mail
Print
Permalink
Citation
EasyBib
EndNote
RefWorks
Delicious
Export RIS
Export BibTeX
This feature requires javascript
Title:
Frequent methylation of RASSF1 and RARB in urine sediments from patients with early stage prostate cancer
Author:
Daniūnaitė, Kristina
;
Berezniakovas, Artūras
;
Jankevičius, Feliksas
;
Laurinavičius, Arvydas
;
Lazutka, Juozas R
;
Jarmalaitė, Sonata
Subjects:
Age Factors
;
Aged
;
Biomarkers, Tumor - urine
;
Biopsy
;
DNA Methylation
;
Glutathione S-Transferase pi - genetics
;
Glutathione S-Transferase pi - urine
;
Humans
;
Male
;
Middle Aged
;
Neoplasm Staging
;
Predictive Value of Tests
;
Prostate - pathology
;
Prostatic Neoplasms - diagnosis
;
Prostatic Neoplasms - genetics
;
Prostatic Neoplasms - pathology
;
Prostatic Neoplasms - urine
;
Real-Time Polymerase Chain Reaction
;
Receptors, Retinoic Acid - genetics
;
Receptors, Retinoic Acid - metabolism
;
Sensitivity and Specificity
;
Tumor Suppressor Proteins - genetics
;
Tumor Suppressor Proteins - urine
Is Part Of:
Medicina (Kaunas, Lithuania), 2011-01, Vol.47 (3), p.147-153
Description:
BACKGROUND. Prostate cancer (PCa) is the second most prevalent malignancy among males, characterized by high mortality rates. Aberrant DNA methylation in promoters of tumor suppressor genes is an early and frequent event during prostate carcinogenesis. Modern techniques allow a sensitive detection of DNA methylation biomarkers in bodily fluids from cancer patients offering a noninvasive tool for PCa monitoring. Our study aimed at the analysis of DNA methylation in urine sediments from PCa patients for the selection of most informative noninvasive biomarkers. MATERIAL AND METHODS. Real-time methylation-specific polymerase chain reaction was used for the detection of methylated RASSF1, RARB, and GSTP1 genes in catheterized urine specimens from 34 patients with biopsy-proven early or medium stage PCa. RESULTS. At least one gene was methylated in urine sediments from 28 cases with PCa, with a sensitivity of the test reaching 82%. RASSF1 was methylated in 71% (24 of 34), RARB in 44% (15 of 34), and GSTP1 in 3% (1 of 34) of the specimens. High level of methylation (≥50%) in RARB and RASSF1 genes was detected in 40% and 20% of cases, respectively. A significant association was observed between high level of RARB methylation and Gleason score (P=0.01), while methylation of at least one gene occurred more frequently in urine DNA of older patients (P=0.02). CONCLUSIONS. Results of our study show a high sensitivity of DNA methylation biomarkers, especially RASSF1 and RARB, for the early and noninvasive detection of PCa.
Publisher:
Switzerland
Language:
English;Lithuanian
Identifier:
ISSN: 1648-9144
EISSN: 1648-9144
DOI: 10.3390/medicina47030020
PMID: 21822036
Source:
MEDLINE
Directory of Open Access Journals
This feature requires javascript
This feature requires javascript
Back to results list
This feature requires javascript
This feature requires javascript
Searching Remote Databases, Please Wait
Searching for
in
scope:(TDTS),scope:(SFX),scope:(TDT),scope:(SEN),primo_central_multiple_fe
Show me what you have so far
This feature requires javascript
This feature requires javascript