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The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle

eLife, 2015-06, Vol.4 [Peer Reviewed Journal]

2015, Nixon et al. This work is licensed under the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/3.0/ ) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2015, Nixon et al 2015 Nixon et al ;ISSN: 2050-084X ;EISSN: 2050-084X ;DOI: 10.7554/eLife.07635 ;PMID: 26090906

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Title:
The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
Author: Nixon, Faye M ; Gutiérrez-Caballero, Cristina ; Hood, Fiona E ; Booth, Daniel G ; Prior, Ian A ; Royle, Stephen J
Subjects: Automation ; cancer ; Cell Biology ; Electron Microscope Tomography ; Electron microscopy ; Fibers ; Gene Expression ; HeLa Cells ; Humans ; Imaging, Three-Dimensional ; Kinases ; Kinetochores - metabolism ; Kinetochores - ultrastructure ; microtubule ; Microtubule-Associated Proteins - metabolism ; Microtubules ; Microtubules - metabolism ; Microtubules - ultrastructure ; Mitosis ; Morphology ; Proteins ; Spindle Apparatus - metabolism ; Structure-function relationships
Is Part Of: eLife, 2015-06, Vol.4
Description: Kinetochore fibers (K-fibers) of the mitotic spindle are force-generating units that power chromosome movement during mitosis. K-fibers are composed of many microtubules that are held together throughout their length. Here, we show, using 3D electron microscopy, that K-fiber microtubules (MTs) are connected by a network of MT connectors. We term this network 'the mesh'. The K-fiber mesh is made of linked multipolar connectors. Each connector has up to four struts, so that a single connector can link up to four MTs. Molecular manipulation of the mesh by overexpression of TACC3 causes disorganization of the K-fiber MTs. Optimal stabilization of K-fibers by the mesh is required for normal progression through mitosis. We propose that the mesh stabilizes K-fibers by pulling MTs together and thereby maintaining the integrity of the fiber. Our work thus identifies the K-fiber meshwork of linked multipolar connectors as a key integrator and determinant of K-fiber structure and function.
Publisher: England: eLife Sciences Publications Ltd
Language: English
Identifier: ISSN: 2050-084X
EISSN: 2050-084X
DOI: 10.7554/eLife.07635
PMID: 26090906
Source: MEDLINE
NCBI PubMed Central（免费）
ROAD: Directory of Open Access Scholarly Resources
ProQuest Central
DOAJ Directory of Open Access Journals

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The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle

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