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Non-contact protein microarray fabrication using a procedure based on liquid bridge formation

Analytical and bioanalytical chemistry, 2009-01, Vol.393 (2), p.591-598 [Peer Reviewed Journal]

Springer-Verlag 2008 ;2009 INIST-CNRS ;ISSN: 1618-2642 ;ISSN: 1618-2650 ;EISSN: 1618-2650 ;DOI: 10.1007/s00216-008-2509-7 ;PMID: 19023564

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Title:
Non-contact protein microarray fabrication using a procedure based on liquid bridge formation
Author: Hartmann, Michael ; Sjödahl, Johan ; Stjernström, Mårten ; Redeby, Johan ; Joos, Thomas ; Roeraade, Johan
Subjects: Analytical Chemistry ; Analytisk kemi ; Beads ; Biochemistry ; Cell Adhesion ; Cell Line ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Deposition ; Dimethyl Sulfoxide ; Exact sciences and technology ; Fluorocarbons ; Fluorocarbons - chemistry ; Food Science ; Format ; General, instrumentation ; Humans ; Hydrolysis ; Immunoassay ; Kemi ; Laboratory Medicine ; Liquids ; Monitoring/Environmental Analysis ; NATURAL SCIENCES ; NATURVETENSKAP ; Original Paper ; Polystyrene resins ; Polystyrenes - chemistry ; Pressure ; Protein Array Analysis - instrumentation ; Protein Array Analysis - methods ; Proteins ; Sensitivity and Specificity ; Serum Albumin - analysis ; Solutions - chemistry ; Spots ; Surface Properties ; Water - chemistry
Is Part Of: Analytical and bioanalytical chemistry, 2009-01, Vol.393 (2), p.591-598
Description: Contemporary microarrayers of contact or non-contact format used in protein microarray fabrication still suffer from a number of problems, e.g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover. In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL –1 human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and a suspension of 5-μm polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures.
Publisher: Berlin/Heidelberg: Springer-Verlag
Language: English
Identifier: ISSN: 1618-2642
ISSN: 1618-2650
EISSN: 1618-2650
DOI: 10.1007/s00216-008-2509-7
PMID: 19023564
Source: MEDLINE
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Non-contact protein microarray fabrication using a procedure based on liquid bridge formation

Springer-Verlag 2008 ;2009 INIST-CNRS ;ISSN: 1618-2642 ;ISSN: 1618-2650 ;EISSN: 1618-2650 ;DOI: 10.1007/s00216-008-2509-7 ;PMID: 19023564

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