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Iμ/I-Raman Determination of Essential Oils’ Constituents from Distillates and Leaf Glands of IOriganum/I Plants

Molecules (Basel, Switzerland), 2023-01, Vol.28 (3) [Peer Reviewed Journal]

COPYRIGHT 2023 MDPI AG ;ISSN: 1420-3049 ;EISSN: 1420-3049 ;DOI: 10.3390/molecules28031221

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  • Title:
    Iμ/I-Raman Determination of Essential Oils’ Constituents from Distillates and Leaf Glands of IOriganum/I Plants
  • Author: Kampasakali, Elli ; Nakas, Alexandros ; Mertzanidis, Dimitrios ; Kokkini, Stella ; Assimopoulou, Andreana N ; Christofilos, Dimitrios
  • Subjects: Analysis ; Chromatography ; Raman spectroscopy
  • Is Part Of: Molecules (Basel, Switzerland), 2023-01, Vol.28 (3)
  • Description: A novel, inexpensive and simple experimental setup for collecting μ-Raman spectra of volatile liquids in very small quantities was developed. It takes advantage of capillary forces to detain minute volatile liquid volumes. Spectra of volatile and even scattering or absorbing media can be measured more effectively. The method is used to facilitate the collection of intensity-consistent Raman spectra from a series of reference compounds present in Origanum essential oils, in order to quantify their constituents by multiple linear regression. Wild grown Origanum plants, collected from five different regions in Greece and taxonomically identified as O. onites, O. vulgare subsp. hirtum and O. vulgare subsp. vulgare, were appropriately distilled to acquire their essential oils. Comparison of the Raman results with those from headspace gas chromatography–mass spectrometry (HS GC-MS) confirmed the successful relative quantification of the most abundant essential oil constituents, highlighting the similarities and differences of the three Origanum taxa examined. Finally, it is demonstrated that directly measuring the leaf peltate glandular hairs yields exploitable results to identify the main components of the essential oil they contain, underlining the potential of in situ (field or industry) measurements utilizing microscope-equipped portable Raman spectrometers.
  • Publisher: MDPI AG
  • Language: English
  • Identifier: ISSN: 1420-3049
    EISSN: 1420-3049
    DOI: 10.3390/molecules28031221
  • Source: GFMER Free Medical Journals
    PubMed Central
    Alma/SFX Local Collection
    ProQuest Central
    DOAJ Directory of Open Access Journals
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