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Reduced Plasma RNA Integrity in Nasopharyngeal Carcinoma Patients
Clinical cancer research, 2006-04, Vol.12 (8), p.2512-2516
[Peer Reviewed Journal]
2006 INIST-CNRS ;ISSN: 1078-0432 ;EISSN: 1557-3265 ;DOI: 10.1158/1078-0432.CCR-05-2572 ;PMID: 16638860
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Title:
Reduced Plasma RNA Integrity in Nasopharyngeal Carcinoma Patients
Author:
WONG, Blenda C. K
;
CHAN, K. C. Allen
;
CHAN, Anthony T. C
;
LEUNG, Sing-Fai
;
CHAN, Lisa Y. S
;
CHOW, Katherine C. K
;
LO, Y. M. Dennis
Subjects:
Adult
;
Antineoplastic agents
;
Biological and medical sciences
;
Female
;
Glyceraldehyde-3-Phosphate Dehydrogenases - genetics
;
Humans
;
Male
;
Medical sciences
;
Middle Aged
;
nasopharyngeal carcinoma
;
Nasopharyngeal Neoplasms - blood
;
Nasopharyngeal Neoplasms - drug therapy
;
Nasopharyngeal Neoplasms - pathology
;
noninvasive
;
Otorhinolaryngology. Stomatology
;
Pharmacology. Drug treatments
;
plasma
;
Reverse Transcriptase Polymerase Chain Reaction
;
RNA integrity
;
RNA Stability - radiation effects
;
RNA, Messenger - blood
;
RNA, Messenger - genetics
;
RNA, Neoplasm - blood
;
RNA, Neoplasm - genetics
;
Tumors
;
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
Is Part Of:
Clinical cancer research, 2006-04, Vol.12 (8), p.2512-2516
Description:
Purpose: Recent research has shown the feasibility of detecting cell-free RNA markers in human subjects. As elevated RNase activity has previously been described in the circulation of cancer patients, we hypothesized that cancer patients may have reduced plasma RNA integrity. In this study, we used nasopharyngeal carcinoma (NPC) as a model system to test this hypothesis. Experimental Design: Plasma RNA integrity was determined using the ratio of the concentrations of transcript sequences corresponding to the 3′ to those from the 5′ end of a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ). Transcript concentrations were measured using real-time quantitative reverse transcription-PCR assays targeting the 5′ and 3′ regions. We analyzed the plasma RNA integrity in 49 untreated NPC patients and 53 healthy controls. We also assessed the plasma samples from 19 NPC patients before and after radiotherapy to further show the clinical potential of this marker. Results: The 3′ to 5′ GAPDH ratio was significantly lower in the plasma of untreated NPC patients when compared with healthy controls (0.0252 versus 0.0485, P = 0.024). Statistical analysis showed that plasma GAPDH ratio was correlated with tumor stage but not with sex and age. Moreover, 14 of 19 NPC patients (74%) showed significant increase in the plasma GAPDH ratio following radiotherapy ( P = 0.003). All of these patients were in clinical remission after treatment. Conclusions: Our findings suggest that NPC is associated with disturbances in the integrity of cell-free circulating RNA, raising the possibility that measurement of plasma RNA integrity may serve as a useful marker for the diagnosis and monitoring of malignant diseases.
Publisher:
Philadelphia, PA: American Association for Cancer Research
Language:
English
Identifier:
ISSN: 1078-0432
EISSN: 1557-3265
DOI: 10.1158/1078-0432.CCR-05-2572
PMID: 16638860
Source:
GFMER Free Medical Journals
MEDLINE
Alma/SFX Local Collection
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