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Rapid and sensitive biosensor for Salmonella

Biosensors & bioelectronics, 2000-06, Vol.15 (3), p.135-141 [Peer Reviewed Journal]

2000 Elsevier Science S.A. ;2000 INIST-CNRS ;ISSN: 0956-5663 ;EISSN: 1873-4235 ;DOI: 10.1016/S0956-5663(00)00067-1 ;PMID: 11286330

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Title:
Rapid and sensitive biosensor for Salmonella
Author: Pathirana, S.T ; Barbaree, J ; Chin, B.A ; Hartell, M.G ; Neely, W.C ; Vodyanoy, V
Subjects: Acoustic wave device ; Animals ; Antibodies, Bacterial ; Antigen–antibody monolayers ; Bacteriological Techniques ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensing Techniques - statistics & numerical data ; Biosensors ; Biotechnology ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Langmuir–Blodgett immobilization ; Methods. Procedures. Technologies ; Salmonella typhimurium ; Salmonella typhimurium - immunology ; Salmonella typhimurium - isolation & purification ; Sensitivity and Specificity ; Sensor ; Time Factors ; Various methods and equipments
Is Part Of: Biosensors & bioelectronics, 2000-06, Vol.15 (3), p.135-141
Description: The rapid and sensitive detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir–Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated. The binding of bacteria to the surface changed the crystal resonance parameters; these were quantified by the output voltage of the sensor instrumentation. The sensor had a lower detection limit of a few hundred cells/ml, and a response time of <100 s over the range of 10 2–10 10 cells/ml. The sensor response was linear between bacterial concentrations of 10 2–10 7 cells/ml, with a sensitivity of 18 mV/decade. The binding of bacteria was specific with two binding sites needed to bind a single cell. The sensors preserve ∼75% of their sensitivity over a period of 32 days.
Publisher: Lausanne: Elsevier B.V
Language: English
Identifier: ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/S0956-5663(00)00067-1
PMID: 11286330
Source: MEDLINE
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Rapid and sensitive biosensor for Salmonella

2000 Elsevier Science S.A. ;2000 INIST-CNRS ;ISSN: 0956-5663 ;EISSN: 1873-4235 ;DOI: 10.1016/S0956-5663(00)00067-1 ;PMID: 11286330

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