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Lung exposure to nanoparticles modulates an asthmatic response in a mouse model

The European respiratory journal, 2011-02, Vol.37 (2), p.299 [Peer Reviewed Journal]

EISSN: 1399-3003 ;DOI: 10.1183/09031936.00168509 ;PMID: 20530043

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Title:
Lung exposure to nanoparticles modulates an asthmatic response in a mouse model
Author: Hussain, S ; Vanoirbeek, J A J ; Luyts, K ; De Vooght, V ; Verbeken, E ; Thomassen, L C J ; Martens, J A ; Dinsdale, D ; Boland, S ; Marano, F ; Nemery, B ; Hoet, P H M
Subjects: Animals ; Asthma - chemically induced ; Asthma - physiopathology ; Bronchial Hyperreactivity ; Bronchoalveolar Lavage Fluid ; Disease Models, Animal ; Eosinophils ; Gold - adverse effects ; Immunoglobulin E - blood ; Lung - physiopathology ; Macrophages ; Male ; Mice ; Mice, Inbred BALB C ; Nanoparticles - adverse effects ; Neutrophils ; Pulmonary Edema - chemically induced ; Pulmonary Edema - physiopathology ; Titanium - adverse effects ; Toluene 2,4-Diisocyanate - toxicity
Is Part Of: The European respiratory journal, 2011-02, Vol.37 (2), p.299
Description: The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO₂) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanate-induced asthma. On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone-olive oil (AOO) on the dorsum of both ears (20 μL). On day 14, the mice were oropharyngeally dosed with 40 μL of a NP suspension (0.4 mg·mL⁻¹ (∼0.8 mg·kg⁻¹) TiO₂ or Au). 1 day later (day 15), the mice received an oropharyngeal challenge with 0.01% TDI (20 μL). On day 16, airway hyperreactivity (AHR), bronchoalveolar lavage (BAL) cell and cytokine analysis, lung histology, and total serum immunoglobulin E were assessed. NP exposure in sensitised mice led to a two- (TiO₂) or three-fold (Au) increase in AHR, and a three- (TiO₂) or five-fold (Au) increase in BAL total cell counts, mainly comprising neutrophils and macrophages. The NPs taken up by BAL macrophages were identified by energy dispersive X-ray spectroscopy. Histological analysis revealed increased oedema, epithelial damage and inflammation. In conclusion, these results show that a low, intrapulmonary doses of TiO₂ or Au NPs can aggravate pulmonary inflammation and AHR in a mouse model of diisocyanate-induced asthma.
Publisher: England
Language: English
Identifier: EISSN: 1399-3003
DOI: 10.1183/09031936.00168509
PMID: 20530043
Source: MEDLINE
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Lung exposure to nanoparticles modulates an asthmatic response in a mouse model

EISSN: 1399-3003 ;DOI: 10.1183/09031936.00168509 ;PMID: 20530043

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