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Transcriptome-Wide Changes in Chlamydomonas reinhardtii Gene Expression Regulated by Carbon Dioxide and the CO₂-Concentrating Mechanism Regulator CIA5/CCM1

The Plant cell, 2012-05, Vol.24 (5), p.1876-1893 [Peer Reviewed Journal]

2012 American Society of Plant Biologists ;Copyright American Society of Plant Biologists May 2012 ;2012 American Society of Plant Biologists. All rights reserved. 2012 ;ISSN: 1040-4651 ;EISSN: 1532-298X ;DOI: 10.1105/tpc.112.097949 ;PMID: 22634760

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Title:
Transcriptome-Wide Changes in Chlamydomonas reinhardtii Gene Expression Regulated by Carbon Dioxide and the CO₂-Concentrating Mechanism Regulator CIA5/CCM1
Author: Fang, Wei ; Si, Yaqing ; Douglass, Stephen ; Casero, David ; Merchant, Sabeeha S. ; Pellegrini, Matteo ; Ladunga, Istvan ; Liu, Peng ; Spalding, Martin H.
Subjects: Acclimatization ; Calvin cycle ; Carbon Dioxide - pharmacology ; Chlamydomonas reinhardtii - drug effects ; Chlamydomonas reinhardtii - genetics ; Chloroplasts ; Datasets ; Gene expression ; Gene Expression - drug effects ; Gene Expression - genetics ; Gene expression regulation ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - genetics ; Genes ; Molecular Sequence Data ; Plant cells ; Protein metabolism ; Transcriptome - drug effects ; Transcriptome - genetics ; Transcriptomes
Is Part Of: The Plant cell, 2012-05, Vol.24 (5), p.1876-1893
Description: We used RNA sequencing to query the Chlamydomonas reinhardtii transcriptome for regulation by CO₂ and by the transcription regulator CIA5 (CCM1). Both CO₂ and CIA5 are known to play roles in acclimation to low CO₂ and in induction of an essential CO₂-concentrating mechanism (CCM), but less is known about their interaction and impact on the whole transcriptome. Our comparison of the transcriptome of a wild type versus a cia5 mutant strain under three different CO₂ conditions, high CO₂ (5%), low CO₂ (0.03 to 0.05%), and very low CO₂ (< 0.02%), provided an entry into global changes in the gene expression patterns occurring in response to the interaction between CO₂ and CIA5. We observed a massive impact of CIA5 and CO₂ on the transcriptome, affecting almost 25% of all Chlamydomonas genes, and we discovered an array of gene clusters with distinctive expression patterns that provide insight into the regulatory interaction between CIA5 and CO₂. Several individual clusters respond primarily to either CIA5 or CO₂, providing access to genes regulated by one factor but decoupled from the other. Three distinct clusters clearly associated with CCM-related genes may represent a rich source of candidates for new CCM components, including a small cluster of genes encoding putative inorganic carbon transporters.
Publisher: England: American Society of Plant Biologists
Language: English
Identifier: ISSN: 1040-4651
EISSN: 1532-298X
DOI: 10.1105/tpc.112.097949
PMID: 22634760
Source: GFMER Free Medical Journals
MEDLINE
ProQuest Central

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Transcriptome-Wide Changes in Chlamydomonas reinhardtii Gene Expression Regulated by Carbon Dioxide and the CO₂-Concentrating Mechanism Regulator CIA5/CCM1

2012 American Society of Plant Biologists ;Copyright American Society of Plant Biologists May 2012 ;2012 American Society of Plant Biologists. All rights reserved. 2012 ;ISSN: 1040-4651 ;EISSN: 1532-298X ;DOI: 10.1105/tpc.112.097949 ;PMID: 22634760

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