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Expression of Adipocyte Biomarkers in a Primary Cell Culture Models Reflects Preweaning Adipobiology

The Journal of biological chemistry, 2014-06, Vol.289 (26), p.18478-18488 [Peer Reviewed Journal]

2014 © 2014 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. ;2014 by The American Society for Biochemistry and Molecular Biology, Inc. ;2014 by The American Society for Biochemistry and Molecular Biology, Inc. 2014 ;ISSN: 0021-9258 ;EISSN: 1083-351X ;DOI: 10.1074/jbc.M114.555821 ;PMID: 24808178

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  • Title:
    Expression of Adipocyte Biomarkers in a Primary Cell Culture Models Reflects Preweaning Adipobiology
  • Author: Chu, Dinh-Toi ; Malinowska, Elzbieta ; Gawronska-Kozak, Barbara ; Kozak, Leslie P.
  • Subjects: Adipocytes - cytology ; Adipocytes - metabolism ; Adipogenesis ; Adipose Tissue - cytology ; Adipose Tissue - growth & development ; Adipose Tissue - metabolism ; Animals ; Biomarkers - metabolism ; Cells, Cultured ; Energy Metabolism ; Female ; Gene Expression ; Humans ; Lipid Metabolism ; Mesenchymal Stem Cells (MSCs) ; Mesoderm-specific Transcript ; Metabolism ; Mice ; Mice, Inbred C57BL ; Microarray ; Models, Biological ; Obesity ; Primary Adipocyte Cultures ; Sfrp5
  • Is Part Of: The Journal of biological chemistry, 2014-06, Vol.289 (26), p.18478-18488
  • Description: A cohort of genes was selected to characterize the adipogenic phenotype in primary cell cultures from three tissue sources. We compared the quantitative expression of biomarkers in culture relative to their expression in vivo because the mere presence or absence of expression is minimally informative. Although all biomarkers analyzed have biochemical functions in adipocytes, the expression of some of the biomarkers varied enormously in culture relative to their expression in the adult fat tissues in vivo, i.e. inguinal fat for white adipocytes and brite cells, interscapular brown adipose tissue for brown adipocytes, and ear mesenchymal stem cells for white adipocytes from adult mice. We propose that the pattern of expression in vitro does not reflect gene expression in the adult mouse; rather it is predominantly the expression pattern of adipose tissue of the developing mouse between birth and weaning. The variation in gene expression among fat depots in both human and rodent has been an extensively studied phenomenon, and as recently reviewed, it is related to subphenotypes associated with immune function, the inflammatory response, fat depot blood flow, and insulin sensitivity. We suggest that adipose tissue biology in the period from birth to weaning is not just a staging platform for the emergence of adult white fat but that it has properties to serve the unique needs of energy metabolism in the newborn. A case in point is the differentiation of brite cells that occurs during this period followed by their involution immediately following weaning. Background: Obesity depends upon a balance between adipocytes associated with thermogenesis and fat storage. Results: Molecular biomarkers characterize primary adipocyte cultures from different adipose depots. Conclusion: Only adipocytes from ear mesenchymal stem cells (EMSCs) failed to express the brown fat biomarkers, and overall gene expression resembled that of neonatal animals. Significance: Metabolism of fat tissue in the preweaning period is distinct from that of adulthood.
  • Publisher: United States: Elsevier Inc
  • Language: English
  • Identifier: ISSN: 0021-9258
    EISSN: 1083-351X
    DOI: 10.1074/jbc.M114.555821
    PMID: 24808178
  • Source: MEDLINE
    PubMed Central
    Alma/SFX Local Collection
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