Language:

Oxygen Generating Polymeric Nano Fibers That Stimulate Angiogenesis and Show Efficient Wound Healing in a Diabetic Wound Model

International journal of nanomedicine, 2020-01, Vol.15, p.3511-3522 [Peer Reviewed Journal]

2020 Zehra et al. ;COPYRIGHT 2020 Dove Medical Press Limited ;2020. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2020 Zehra et al. 2020 Zehra et al. ;ISSN: 1178-2013 ;ISSN: 1176-9114 ;EISSN: 1178-2013 ;DOI: 10.2147/IJN.S248911 ;PMID: 32547010

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Oxygen Generating Polymeric Nano Fibers That Stimulate Angiogenesis and Show Efficient Wound Healing in a Diabetic Wound Model
Author: Zehra, Mubashra ; Zubairi, Waliya ; Hasan, Anwarul ; Butt, Hira ; Ramzan, Amna ; Azam, Maryam ; Mehmood, Azra ; Falahati, Mojtaba ; Chaudhry, Aqif Anwar ; Rehman, Ihtesham Ur ; Yar, Muhammad
Subjects: Amputation ; Analysis ; Angiogenesis ; Animals ; Bandages ; Biological Assay ; Biomedical materials ; Cell adhesion & migration ; Chorioallantoic Membrane - drug effects ; Chorioallantoic Membrane - metabolism ; Diabetes ; Diabetes Mellitus - pathology ; Diabetes Mellitus, Experimental - pathology ; diabetic wound healing ; Disease Models, Animal ; Eggs ; electrospinning ; Fibroblasts ; Foot diseases ; Fourier transforms ; Gene expression ; Gene Expression Regulation - drug effects ; Humans ; Hypoxia ; Infrared spectroscopy ; Male ; Molecular biology ; Nanofibers - chemistry ; Nanofibers - ultrastructure ; Neovascularization, Physiologic - drug effects ; Original Research ; Oxygen - chemistry ; oxygenation ; Polycaprolactone ; Polyesters - pharmacology ; polymeric nanofibers ; Polymers ; Protein expression ; Rats, Sprague-Dawley ; Reproducibility of Results ; Skin ; Skin - pathology ; skin regeneration ; Spectroscopy, Fourier Transform Infrared ; Spectrum analysis ; Studies ; Tissue engineering ; Ulcers ; Wound care ; Wound healing ; Wound Healing - drug effects ; X-ray spectroscopy
Is Part Of: International journal of nanomedicine, 2020-01, Vol.15, p.3511-3522
Description: Diabetic wounds are challenging to treat due to a wide range of pathophysiological changes. Hypoxia is one of the predominant contributing factors of poor vascularization and chronicity in diabetic wounds. This study was designed to develop polycaprolactone (PCL)-based oxygen-releasing electrospun wound dressings and evaluate their efficacy for improved full thickness wound healing in diabetic rats. PCL-based oxygen releasing wound dressings were made using electrospinning technology. The developed dressings were characterized in terms of physical as well as biological properties both in vitro and in vivo. E-spun nanofibrous dressings were physically characterized with scanning electron microscopy, Fourier-transform infrared spectroscopy, and Energy-dispersive X-ray spectroscopy. To study the likely impact of the fabricated wound dressings in hypoxic conditions, HIF-1α expression analysis was carried out both at gene and protein levels. Wound dressings were further evaluated for their healing potential for extensive wounds in diabetic rat models. The experimental results showed that the developed dressings were capable of continuously generating oxygen for up to 10 days. Cell studies further confirmed pronounced expression of HIF-1α at gene and protein levels in cells seeded on PCL-sodium percarbonate (SPC) and PCL scaffolds compared with the cells cultured on a tissue culture plate. Chorioallantoic membrane assay revealed the supportive role of oxygen releasing dressings on angiogenesis compared to the control group. Histological assessment of the regenerated skin tissues proved that full thickness wounds covered with SPC loaded PCL dressings had a comparatively better vascularized and compact extracellular matrix with completely covered thick epithelium. The developed oxygen generating polymeric nanofibrous wound dressings could potentially be used as an envisioned approach for the efficient recovery of chronic diabetic wounds.
Publisher: New Zealand: Dove Medical Press Limited
Language: English
Identifier: ISSN: 1178-2013
ISSN: 1176-9114
EISSN: 1178-2013
DOI: 10.2147/IJN.S248911
PMID: 32547010
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
DOVE Medical Press Journals
ProQuest Central
DOAJ Directory of Open Access Journals

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Oxygen Generating Polymeric Nano Fibers That Stimulate Angiogenesis and Show Efficient Wound Healing in a Diabetic Wound Model

Searching Remote Databases, Please Wait