Language:

Skin-on-a-chip model simulating inflammation, edema and drug-based treatment

Scientific reports, 2016-11, Vol.6 (1), p.37471, Article 37471 [Peer Reviewed Journal]

Copyright Nature Publishing Group Nov 2016 ;Copyright © 2016, The Author(s) 2016 The Author(s) ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep37471 ;PMID: 27869150

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Skin-on-a-chip model simulating inflammation, edema and drug-based treatment
Author: Wufuer, Maierdanjiang ; Lee, GeonHui ; Hur, Woojune ; Jeon, Byoungjun ; Kim, Byung Jun ; Choi, Tae Hyun ; Lee, SangHoon
Subjects: Cell culture ; Cosmetics ; Cosmetics industry ; Dexamethasone - pharmacology ; Dexamethasone - therapeutic use ; Edema ; Edema - drug therapy ; Edema - pathology ; Endothelium ; Human Umbilical Vein Endothelial Cells ; Humans ; Immunohistochemistry ; Inflammation ; Inflammation - drug therapy ; Inflammation - pathology ; Lab-On-A-Chip Devices ; Microfluidics ; Models, Biological ; Optical Imaging ; Permeability ; Protective Agents - pharmacology ; Protective Agents - therapeutic use ; Skin ; Skin - pathology ; Skin diseases ; Tight junctions ; Tight Junctions - drug effects ; Tight Junctions - metabolism ; Toxicity ; Toxicity testing ; Tumor Necrosis Factor-alpha - pharmacology ; Tumor necrosis factor-α
Is Part Of: Scientific reports, 2016-11, Vol.6 (1), p.37471, Article 37471
Description: Recent advances in microfluidic cell cultures enable the construction of in vitro human skin models that can be used for drug toxicity testing, disease study. However, current in vitro skin model have limitations to emulate real human skin due to the simplicity of model. In this paper, we describe the development of 'skin-on-a-chip' to mimic the structures and functional responses of the human skin. The proposed model consists of 3 layers, on which epidermal, dermal and endothelial components originated from human, were cultured. The microfluidic device was designed for co-culture of human skin cells and each layer was separated by using porous membranes to allow interlayer communication. Skin inflammation and edema were induced by applying tumor necrosis factor alpha on dermal layer to demonstrate the functionality of the system. The expression levels of proinflammatory cytokines were analyzed to illustrate the feasibility. In addition, we evaluated the efficacy of therapeutic drug testing model using our skin chip. The function of skin barrier was evaluated by staining tight junctions and measuring a permeability of endothelium. Our results suggest that the skin-on-a-chip model can potentially be used for constructing in vitro skin disease models or for testing the toxicity of cosmetics or drugs.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 2045-2322
EISSN: 2045-2322
DOI: 10.1038/srep37471
PMID: 27869150
Source: Open Access: PubMed Central
AUTh Library subscriptions: ProQuest Central
MEDLINE
DOAJ Directory of Open Access Journals

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Skin-on-a-chip model simulating inflammation, edema and drug-based treatment

Copyright Nature Publishing Group Nov 2016 ;Copyright © 2016, The Author(s) 2016 The Author(s) ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep37471 ;PMID: 27869150

Searching Remote Databases, Please Wait