Language:

COVID-19 survival associates with the immunoglobulin response to the SARS-CoV-2 spike receptor binding domain

The Journal of clinical investigation, 2020-12, Vol.130 (12), p.6366-6378 [Peer Reviewed Journal]

COPYRIGHT 2020 American Society for Clinical Investigation ;COPYRIGHT 2020 American Society for Clinical Investigation ;2020 American Society for Clinical Investigation 2020 American Society for Clinical Investigation ;ISSN: 0021-9738 ;EISSN: 1558-8238 ;DOI: 10.1172/JCI142804 ;PMID: 32991329

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
COVID-19 survival associates with the immunoglobulin response to the SARS-CoV-2 spike receptor binding domain
Author: Secchi, Massimiliano ; Bazzigaluppi, Elena ; Brigatti, Cristina ; Marzinotto, Ilaria ; Tresoldi, Cristina ; Rovere-Querini, Patrizia ; Poli, Andrea ; Castagna, Antonella ; Scarlatti, Gabriella ; Zangrillo, Alberto ; Ciceri, Fabio ; Piemonti, Lorenzo ; Lampasona, Vito
Subjects: Aged ; Antibodies, Viral - immunology ; Antigens, Viral - immunology ; Care and treatment ; Clinical Medicine ; Coronaviruses ; COVID-19 ; COVID-19 - immunology ; COVID-19 - mortality ; Development and progression ; Epitopes - immunology ; Female ; Follow-Up Studies ; Health aspects ; Humans ; Immune response ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulins ; Influenza ; Influenza viruses ; Lectins ; Male ; Medical research ; Medicine, Experimental ; Middle Aged ; Patient outcomes ; Physiological aspects ; Protein binding ; Protein Domains ; SARS-CoV-2 - immunology ; Severe acute respiratory syndrome ; Spike Glycoprotein, Coronavirus
Is Part Of: The Journal of clinical investigation, 2020-12, Vol.130 (12), p.6366-6378
Description: BACKGROUNDSerological assays are of critical importance to investigate correlates of response and protection in coronavirus disease 2019 (COVID-19), to define previous exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in populations, and to verify the development of an adaptive immune response in infected individuals.METHODSWe studied 509 patients confirmed to have COVID-19 from the San Raffaele Hospital of Milan and 480 samples of prepandemic organ donor sera collected in 2010-2012. Using fluid-phase luciferase immune precipitation (LIPS) assays, we characterized IgG, IgM, and IgA antibodies to the spike receptor binding domain (RBD), S1+S2, nucleocapsid, and ORF6 to ORF10 of SARS-CoV-2, to the HCoV-OC43 and HCoV-HKU1 betacoronaviruses spike S2, and the H1N1Ca2009 flu virus hemagglutinin. Sequential samples at 1 and 3 months after hospital discharge were also tested for SARS-CoV-2 RBD antibodies in 95 patients.RESULTSAntibodies developed rapidly against multiple SARS-CoV-2 antigens in 95% of patients by 4 weeks after symptom onset and IgG to the RBD increased until the third month of follow-up. We observed a major synchronous expansion of antibodies to the HCoV-OC43 and HCoV-HKU1 spike S2. A likely coinfection with influenza was neither linked to a more severe presentation of the disease nor to a worse outcome. Of the measured antibody responses, positivity for IgG against the SARS-CoV-2 spike RBD was predictive of survival.CONCLUSIONThe measurement of antibodies to selected epitopes of SARS-CoV-2 antigens can offer a more accurate assessment of the humoral response in patients and its impact on survival. The presence of partially cross-reactive antibodies with other betacoronaviruses is likely to impact on serological assay specificity and interpretation.TRIAL REGISTRATIONCOVID-19 Patients Characterization, Biobank, Treatment Response and Outcome Predictor (COVID-BioB). ClinicalTrials.gov identifier: NCT04318366.FUNDINGIRCCS Ospedale San Raffaele and Università Vita Salute San Raffaele.
Publisher: United States: American Society for Clinical Investigation
Language: English
Identifier: ISSN: 0021-9738
EISSN: 1558-8238
DOI: 10.1172/JCI142804
PMID: 32991329
Source: Open Access: PubMed Central
Geneva Foundation Free Medical Journals at publisher websites
AUTh Library subscriptions: ProQuest Central
MEDLINE

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

COVID-19 survival associates with the immunoglobulin response to the SARS-CoV-2 spike receptor binding domain

COPYRIGHT 2020 American Society for Clinical Investigation ;COPYRIGHT 2020 American Society for Clinical Investigation ;2020 American Society for Clinical Investigation 2020 American Society for Clinical Investigation ;ISSN: 0021-9738 ;EISSN: 1558-8238 ;DOI: 10.1172/JCI142804 ;PMID: 32991329

Searching Remote Databases, Please Wait