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Naturally Occurring Human Urinary Peptides for Use in Diagnosis of Chronic Kidney Disease

Molecular & cellular proteomics, 2010-11, Vol.9 (11), p.2424-2437 [Peer Reviewed Journal]

2010 © 2010 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. ;2010 by The American Society for Biochemistry and Molecular Biology, Inc. ;ISSN: 1535-9476 ;EISSN: 1535-9484 ;DOI: 10.1074/mcp.M110.001917 ;PMID: 20616184

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Title:
Naturally Occurring Human Urinary Peptides for Use in Diagnosis of Chronic Kidney Disease
Author: Good, David M. ; Zürbig, Petra ; Argilés, Àngel ; Bauer, Hartwig W. ; Behrens, Georg ; Coon, Joshua J. ; Dakna, Mohammed ; Decramer, Stéphane ; Delles, Christian ; Dominiczak, Anna F. ; Ehrich, Jochen H.H. ; Eitner, Frank ; Fliser, Danilo ; Frommberger, Moritz ; Ganser, Arnold ; Girolami, Mark A. ; Golovko, Igor ; Gwinner, Wilfried ; Haubitz, Marion ; Herget-Rosenthal, Stefan ; Jankowski, Joachim ; Jahn, Holger ; Jerums, George ; Julian, Bruce A. ; Kellmann, Markus ; Kliem, Volker ; Kolch, Walter ; Krolewski, Andrzej S. ; Luppi, Mario ; Massy, Ziad ; Melter, Michael ; Neusüss, Christian ; Novak, Jan ; Peter, Karlheinz ; Rossing, Kasper ; Rupprecht, Harald ; Schanstra, Joost P. ; Schiffer, Eric ; Stolzenburg, Jens-Uwe ; Tarnow, Lise ; Theodorescu, Dan ; Thongboonkerd, Visith ; Vanholder, Raymond ; Weissinger, Eva M. ; Mischak, Harald ; Schmitt-Kopplin, Philippe
Subjects: Adult ; Aged ; Biomarkers - urine ; Databases, Factual ; Electrophoresis, Capillary - methods ; Female ; Humans ; Kidney Failure, Chronic - diagnosis ; Kidney Failure, Chronic - urine ; Male ; Mass Spectrometry - methods ; Middle Aged ; Peptides - urine ; Proteomics - methods ; ROC Curve ; Young Adult
Is Part Of: Molecular & cellular proteomics, 2010-11, Vol.9 (11), p.2424-2437
Description: Because of its availability, ease of collection, and correlation with physiology and pathology, urine is an attractive source for clinical proteomics/peptidomics. However, the lack of comparable data sets from large cohorts has greatly hindered the development of clinical proteomics. Here, we report the establishment of a reproducible, high resolution method for peptidome analysis of naturally occurring human urinary peptides and proteins, ranging from 800 to 17,000 Da, using samples from 3,600 individuals analyzed by capillary electrophoresis coupled to MS. All processed data were deposited in an Structured Query Language (SQL) database. This database currently contains 5,010 relevant unique urinary peptides that serve as a pool of potential classifiers for diagnosis and monitoring of various diseases. As an example, by using this source of information, we were able to define urinary peptide biomarkers for chronic kidney diseases, allowing diagnosis of these diseases with high accuracy. Application of the chronic kidney disease-specific biomarker set to an independent test cohort in the subsequent replication phase resulted in 85.5% sensitivity and 100% specificity. These results indicate the potential usefulness of capillary electrophoresis coupled to MS for clinical applications in the analysis of naturally occurring urinary peptides.
Publisher: United States: Elsevier Inc
Language: English
Identifier: ISSN: 1535-9476
EISSN: 1535-9484
DOI: 10.1074/mcp.M110.001917
PMID: 20616184
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
Alma/SFX Local Collection

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Naturally Occurring Human Urinary Peptides for Use in Diagnosis of Chronic Kidney Disease

2010 © 2010 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. ;2010 by The American Society for Biochemistry and Molecular Biology, Inc. ;ISSN: 1535-9476 ;EISSN: 1535-9484 ;DOI: 10.1074/mcp.M110.001917 ;PMID: 20616184

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