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Human ASIC3 channel dynamically adapts its activity to sense the extracellular pH in both acidic and alkaline directions

Proceedings of the National Academy of Sciences - PNAS, 2012-08, Vol.109 (32), p.13124-13129 [Peer Reviewed Journal]

copyright © 1993-2008 National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Aug 7, 2012 ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.1120350109 ;PMID: 22829666

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Title:
Human ASIC3 channel dynamically adapts its activity to sense the extracellular pH in both acidic and alkaline directions
Author: Delaunay, Anne ; Gasull, Xavier ; Salinas, Miguel ; Noël, Jacques ; Friend, Valérie ; Lingueglia, Eric ; Deval, Emmanuel
Subjects: Acid Sensing Ion Channels ; Acidification ; acidosis ; alkalinization ; Animals ; Biological Sciences ; brain ; Calcium ; Cells ; Cercopithecus aethiops ; Cloning ; COS Cells ; Extracellular Fluid - chemistry ; Fluorescence ; ganglia ; Humans ; Hydrogen-Ion Concentration ; Ion channels ; Life Sciences ; membrane potential ; Membrane Potentials - physiology ; Messenger RNA ; Nerve tissue ; Neurons ; Neurons - metabolism ; Neurons - physiology ; Neurons and Cognition ; Pain ; Patch-Clamp Techniques ; Ribonucleic acid ; RNA ; Rodents ; sensory neurons ; Sodium Channels - metabolism ; spinal cord ; Tissues
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2012-08, Vol.109 (32), p.13124-13129
Description: In rodent sensory neurons, acid-sensing ion channel 3 (ASIC3) has recently emerged as a particularly important sensor of nonadaptive pain associated with tissue acidosis. However, little is known about the human ASIC3 channel, which includes three splice variants differing in their C-terminal domain (hASIC3a, hASIC3b, and hASIC3c). hASIC3a transcripts represent the main mRNAs expressed in both peripheral and central neuronal tissues (dorsal root ganglia [DRG], spinal cord, and brain), where a small proportion of hASIC3c transcripts is also detected. We show that hASIC3 channels (hASIC3a, hASIC3b, or hASIC3c) are able to directly sense extracellular pH changes not only during acidification (up to pH 5.0), but also during alkalization (up to pH 8.0), an original and inducible property yet unknown. When the external pH decreases, hASIC3 display a transient acid mode with brief activation that is relevant to the classical ASIC currents, as previously described. On the other hand, an external pH increase activates a sustained alkaline mode leading to a constitutive activity at resting pH. Both modes are inhibited by the APETx2 toxin, an ASIC3-type channel inhibitor. The alkaline sensitivity of hASIC3 is an intrinsic property of the channel, which is supported by the extracellular loop and involves two arginines (R68 and R83) only present in the human clone. hASIC3 is thus able to sense the extracellular pH in both directions and therefore to dynamically adapt its activity between pH 5.0 and 8.0, a property likely to participate in the fine tuning of neuronal membrane potential and to neuron sensitization in various pH environments.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.1120350109
PMID: 22829666
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central

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Human ASIC3 channel dynamically adapts its activity to sense the extracellular pH in both acidic and alkaline directions

copyright © 1993-2008 National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Aug 7, 2012 ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.1120350109 ;PMID: 22829666

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