Language:

Rapid cell separation with minimal manipulation for autologous cell therapies

Scientific reports, 2017-02, Vol.7 (1), p.41872-41872, Article 41872 [Peer Reviewed Journal]

Copyright Nature Publishing Group Feb 2017 ;Copyright © 2017, The Author(s) 2017 The Author(s) ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep41872 ;PMID: 28150746

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Rapid cell separation with minimal manipulation for autologous cell therapies
Author: Smith, Alban J ; O'Rorke, Richard D ; Kale, Akshay ; Rimsa, Roberts ; Tomlinson, Matthew J ; Kirkham, Jennifer ; Davies, A Giles ; Wälti, Christoph ; Wood, Christopher D
Subjects: Cell Separation - instrumentation ; Cell Separation - methods ; Cell therapy ; Cells, Cultured ; Conductivity ; Dental pulp ; Dental Pulp - cytology ; Electric fields ; Electrodes ; Electrophoresis - instrumentation ; Electrophoresis - methods ; Humans ; Labelling ; Mesenchymal Stem Cells - cytology ; Mesenchyme ; Microfluidics ; Microfluidics - instrumentation ; Microfluidics - methods ; Regenerative medicine ; Saccharomyces cerevisiae - cytology ; Side effects ; Sonication - instrumentation ; Sonication - methods ; Stromal cells ; Surface acoustic waves
Is Part Of: Scientific reports, 2017-02, Vol.7 (1), p.41872-41872, Article 41872
Description: The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling. Thus a rapid, label-free separation technology that does not affect cell functionality, viability or phenotype is highly desirable. Here, we demonstrate separation of viable from non-viable human stromal cells using remote dielectrophoresis, in which an electric field is coupled into a microfluidic channel using shear-horizontal surface acoustic waves, producing an array of virtual electrodes within the channel. This allows high-throughput dielectrophoretic cell separation in high conductivity, physiological-like fluids, overcoming the limitations of conventional dielectrophoresis. We demonstrate viable/non-viable separation efficacy of >98% in pre-purified mesenchymal stromal cells, extracted from human dental pulp, with no adverse effects on cell viability, or on their subsequent osteogenic capabilities.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 2045-2322
EISSN: 2045-2322
DOI: 10.1038/srep41872
PMID: 28150746
Source: MEDLINE
PubMed Central
Directory of Open Access Journals
ProQuest Central

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Rapid cell separation with minimal manipulation for autologous cell therapies

Copyright Nature Publishing Group Feb 2017 ;Copyright © 2017, The Author(s) 2017 The Author(s) ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep41872 ;PMID: 28150746

Searching Remote Databases, Please Wait