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Abscisic acid, H₂O₂ and nitric oxide interactions mediated cold‐induced S‐adenosylmethionine synthetase in Medicago sativa subsp. falcata that confers cold tolerance through up‐regulating polyamine oxidation

Plant biotechnology journal, 2014-06, Vol.12 (5), p.601-612 [Peer Reviewed Journal]

2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd ;2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. ;Copyright © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd ;ISSN: 1467-7644 ;EISSN: 1467-7652 ;DOI: 10.1111/pbi.12166 ;PMID: 24517136

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Title:
Abscisic acid, H₂O₂ and nitric oxide interactions mediated cold‐induced S‐adenosylmethionine synthetase in Medicago sativa subsp. falcata that confers cold tolerance through up‐regulating polyamine oxidation
Author: Guo, Zhenfei ; Tan, Jiali ; Zhuo, Chunliu ; Wang, Congying ; Xiang, Bin ; Wang, Zengyu
Subjects: abscisic acid ; Abscisic Acid - pharmacology ; acclimation ; Acclimatization - drug effects ; Acclimatization - genetics ; Adaptation, Physiological - drug effects ; Adaptation, Physiological - genetics ; Antioxidants ; Antioxidants - metabolism ; Cloning, Molecular ; Cold ; Cold Temperature ; cold tolerance ; cold treatment ; enzyme activity ; Enzymes ; ethylene ; ethylene production ; Ethylenes - metabolism ; Freezing ; Gene Expression Regulation, Plant - drug effects ; genes ; germplasm ; homeostasis ; hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; leaves ; Medicago sativa - drug effects ; Medicago sativa - enzymology ; Medicago sativa - genetics ; Medicago sativa - physiology ; Medicago sativa subsp. falcata ; Methionine Adenosyltransferase - metabolism ; Nicotiana - genetics ; nitric oxide ; Nitric Oxide - metabolism ; Nucleic Acid Hybridization ; Oxidation ; Oxidation-Reduction - drug effects ; Oxidoreductases Acting on CH-NH Group Donors - metabolism ; Plants, Genetically Modified ; Polyamine Oxidase ; polyamine oxidation ; Polyamines ; Polyamines - metabolism ; putrescine ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; S-Adenosylmethionine - metabolism ; Signal Transduction - drug effects ; Signal Transduction - genetics ; spermidine ; spermine ; S‐adenosylmethionine synthetase ; Time Factors ; tobacco ; Transgenic plants ; Up-Regulation - drug effects
Is Part Of: Plant biotechnology journal, 2014-06, Vol.12 (5), p.601-612
Description: S‐adenosylmethionine synthetase (SAMS) is the key enzyme catalysing the formation of S‐adenosylmethionine (SAM), a precursor of polyamines and ethylene. To investigate the potential role of SAMS in cold tolerance, we isolated MfSAMS1 from the cold‐tolerant germplasm Medicago sativa subsp. falcata and analysed the association of SAM‐derived polyamines with cold tolerance. The expression of MfSAMS1 in leaves was greatly induced by cold, abscisic acid (ABA), H₂O₂ and nitric oxide (NO). Our data revealed that ABA, H₂O₂ and NO interactions mediated the cold‐induced MfSAMS1 expression and cold acclimation in falcata. SAM, putrescine, spermidine and spermine levels, ethylene production and polyamine oxidation were sequentially altered in response to cold, indicating that SAMS‐derived SAM is preferentially used in polyamine synthesis and homeostasis during cold acclimation. Antioxidant enzyme activities were also induced in response to cold and showed correlation with polyamine oxidation. Overexpression of MfSAMS1 in tobacco resulted in elevated SAM levels, but polyamine levels and ethylene production in the transgenic plants were not significantly changed. Compared to the wild type, transgenic plants had increased levels of apoplastic H₂O₂, higher transcript levels of genes involved in polyamine synthesis and oxidation, and higher activities of polyamine oxidation and antioxidant enzymes. The results showed that overexpression of MfSAMS1 promoted polyamine synthesis and oxidation, which in turn improved H₂O₂‐induced antioxidant protection, as a result enhanced tolerance to freezing and chilling stress in transgenic plants. This is the first report demonstrating that SAMS plays an important role in plant tolerance to cold via up‐regulating polyamine oxidation.
Publisher: England: Blackwell Pub
Language: English
Identifier: ISSN: 1467-7644
EISSN: 1467-7652
DOI: 10.1111/pbi.12166
PMID: 24517136
Source: Wiley Online Library Open Access

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Abscisic acid, H₂O₂ and nitric oxide interactions mediated cold‐induced S‐adenosylmethionine synthetase in Medicago sativa subsp. falcata that confers cold tolerance through up‐regulating polyamine oxidation

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