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Simultaneous observation of fluorescent retrogradely labeled neurons and the immunofluorescently labeled fibers apposing them using Fluoro-Gold and antisera labeled with the blue fluorochrome 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

Neuroscience letters, 1987-11, Vol.82 (2), p.121-126 [Peer Reviewed Journal]

1987 ;1988 INIST-CNRS ;ISSN: 0304-3940 ;EISSN: 1872-7972 ;DOI: 10.1016/0304-3940(87)90115-7 ;PMID: 3320819 ;CODEN: NELED5

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Title:
Simultaneous observation of fluorescent retrogradely labeled neurons and the immunofluorescently labeled fibers apposing them using Fluoro-Gold and antisera labeled with the blue fluorochrome 7-amino-4-methylcoumarin-3-acetic acid (AMCA)
Author: Wessendorf, Martin W. ; Appel, Nathan M. ; Elde, Robert
Subjects: 7-Amino-4-methylcoumarin-3-acetic-acid ; AMCA ; Animals ; Biological and medical sciences ; Coumarins ; Fluorescent Antibody Technique ; Fluorescent Dyes ; Fluoro-Gold ; Fundamental and applied biological sciences. Psychology ; Ganglia, Sympathetic - cytology ; Ganglia, Sympathetic - physiology ; Immune Sera - immunology ; Immunofluorescence ; Intermediolateral cell column ; Isolated neuron and nerve. Neuroglia ; Nerve Fibers - physiology ; Neurons - physiology ; Rats ; Retrograde labeling ; Serotonin ; Stilbamidines ; Substance P ; Vertebrates: nervous system and sense organs
Is Part Of: Neuroscience letters, 1987-11, Vol.82 (2), p.121-126
Description: In this paper we describe a method allowing the simultaneous observation of fluorescent retrogradely labeled neurons and the immunofluorescently labeled fibers which appose them. This technique employs the fluorescent dye Fluoro-Gold to label neurons retrogradely, and 7-amino-4-methylcoumarin-3-acetic-acid (AMCA) conjugated to a secondary antiserum as a label for fluorescence immunohistochemistry. Both Fluoro-Gold and AMCA are excited by near-ultraviolet (UV) light, but under UV excitation Fluoro-Gold appears yellow and AMCA appears blue. Thus the two fluorochromes are both visible under a single condition of illumination and can be readily distinguished by color. This allows quick and accurate determination of whether or not immunofluorescent fibers appose retrogradely labeled neurons.
Publisher: Shannon: Elsevier Ireland Ltd
Language: English
Identifier: ISSN: 0304-3940
EISSN: 1872-7972
DOI: 10.1016/0304-3940(87)90115-7
PMID: 3320819
CODEN: NELED5
Source: MEDLINE
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Simultaneous observation of fluorescent retrogradely labeled neurons and the immunofluorescently labeled fibers apposing them using Fluoro-Gold and antisera labeled with the blue fluorochrome 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

1987 ;1988 INIST-CNRS ;ISSN: 0304-3940 ;EISSN: 1872-7972 ;DOI: 10.1016/0304-3940(87)90115-7 ;PMID: 3320819 ;CODEN: NELED5

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