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Dynamic Observation of Oxygenation-Induced Contraction of and Transient Fiber-Network Formation-Disassembly in Cultured Human Brain Microvascular Endothelial Cells

Journal of cerebral blood flow and metabolism, 2003-07, Vol.23 (7), p.821-828 [Peer Reviewed Journal]

2003 The International Society for Cerebral Blood Flow and Metabolism ;2003 INIST-CNRS ;Copyright Nature Publishing Group Jul 2003 ;ISSN: 0271-678X ;EISSN: 1559-7016 ;DOI: 10.1097/01.WCB.0000063992.19746.CA ;PMID: 12843785 ;CODEN: JCBMDN

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  • Title:
    Dynamic Observation of Oxygenation-Induced Contraction of and Transient Fiber-Network Formation-Disassembly in Cultured Human Brain Microvascular Endothelial Cells
  • Author: Inoue, Kouji ; Tomita, Minoru ; Fukuuchi, Yasuo ; Tanahashi, Norio ; Kobari, Masahiro ; Takao, Masaki ; Takeda, Hidetaka ; Yokoyama, Masako
  • Subjects: Biological and medical sciences ; Brain - cytology ; Cell Movement ; Cell Size ; Cells, Cultured ; Cerebrovascular Circulation ; Cytoskeleton - metabolism ; Endothelium, Vascular - cytology ; Endothelium, Vascular - physiology ; Humans ; Medical sciences ; Microcirculation ; Microscopy, Phase-Contrast ; Oxygen - metabolism
  • Is Part Of: Journal of cerebral blood flow and metabolism, 2003-07, Vol.23 (7), p.821-828
  • Description: Oxygenation-induced contraction of nonconfluent cultured human brain microvascular endothelial cells (HBECs, n = 30) was examined by video-enhanced contrast-differential interferential contrast microscopy. After administering a continuous gentle blow of pure oxygen gas to the surface of the medium just above the flattened HBEC, the plasma membrane exhibited tensioning and wrinkling, resulting in a strong contraction of the cell body by 14 ± 7% (P < 0.001). When the cell stopped contracting, transient formation of a fiber network starting from certain spots (possibly adhesion plaques, though these were not visible in the majority of cases) and expanding to the whole cell was observed. The occurrence of fiber network formation was statistically significant (26 of 30 separate cells, P < 0.05). After cessation of oxygen delivery, the observed network of fibers broke up rapidly (in a period of 3.3 ± 1.2 seconds) into small particles of <0.5 μm in diameter, which subsequently fused into the cellular structure. The HBEC completely recovered the control appearance. The sequential process was completed within 30 seconds and was reproduced in individual cells each time that oxygen gas was supplied. The authors conclude that the HBEC strongly contracts in response to a transient oxygenation stimulus, followed by rapid formation/disassembly of a network structure.
  • Publisher: London, England: SAGE Publications
  • Language: English
  • Identifier: ISSN: 0271-678X
    EISSN: 1559-7016
    DOI: 10.1097/01.WCB.0000063992.19746.CA
    PMID: 12843785
    CODEN: JCBMDN
  • Source: MEDLINE
    ProQuest Central
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