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Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales

Journal of antimicrobial chemotherapy, 2020-06, Vol.75 (6), p.1491-1494 [Peer Reviewed Journal]

The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. ;The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. 2020 ;ISSN: 0305-7453 ;EISSN: 1460-2091 ;DOI: 10.1093/JAC/DKAA043 ;PMID: 32083655

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  • Title:
    Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales
  • Author: Bogaerts, Pierre ; Berger, Anne-Sophie ; Evrard, Stéphanie ; Huang, Te-Din
  • Subjects: Original Research
  • Is Part Of: Journal of antimicrobial chemotherapy, 2020-06, Vol.75 (6), p.1491-1494
  • Description: Two commercially available lateral flow immunochromatographic assays (ICAs) for detection of the major carbapenemases were prospectively assessed for the detection of carbapenemases in Enterobacterales: RESIST-4 O.K.N.V. (Coris BioConcept) and NG-Test CARBA 5 (NG Biotech). These two assays were performed prospectively on consecutive Enterobacterales suspected of producing a carbapenemase that were referred to the Belgian National Reference Center for Monitoring Antimicrobial Resistance in Gram-Negative Bacteria between March and June 2018. The intensity of the band corresponding to a carbapenemase for each test was compared using ImageJ software. Of the 161 isolates tested, a carbapenemase was detected in 91 (60 OXA-48-like, 15 VIM, 9 KPC, 5 NDM, 1 IMP and 1 IMP + OXA-48); in the remaining 70, no carbapenemases were detected. For both tests, the results were 100% concordant with the results of the PCR-sequencing reference method. Two IMP producers were only detected by NG-Test CARBA 5 as IMP is not targeted by RESIST-4 O.K.N.V. The mean intensity of the OXA-48, VIM and NDM bands displayed by NG-Test CARBA 5 was 3 to 3.7 times higher than for RESIST-4 O.K.N.V., while the KPC band was on average 1.7 times more intense with RESIST-4 O.K.N.V. RESIST-4 O.K.N.V. and NG-Test CARBA 5 are two efficient assays for identification of the major carbapenemases. NG-Test CARBA 5 offers the advantage of detecting IMP, which remains rare in Western countries.
  • Publisher: England: Oxford University Press
  • Language: English
  • Identifier: ISSN: 0305-7453
    EISSN: 1460-2091
    DOI: 10.1093/JAC/DKAA043
    PMID: 32083655
  • Source: GFMER Free Medical Journals
    Alma/SFX Local Collection
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