skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

Effects of paternal methionine supplementation on sperm DNA methylation and embryo transcriptome in sheep

Environmental epigenetics, 2023, Vol.9 (1), p.dvac029-dvac029 [Peer Reviewed Journal]

The Author(s) 2023. Published by Oxford University Press. 2023 ;The Author(s) 2023. Published by Oxford University Press. ;ISSN: 2058-5888 ;EISSN: 2058-5888 ;DOI: 10.1093/eep/dvac029 ;PMID: 36727109

Full text available

Citations Cited by
  • Title:
    Effects of paternal methionine supplementation on sperm DNA methylation and embryo transcriptome in sheep
  • Author: Townsend, Jessica ; Braz, Camila U ; Taylor, Todd ; Khatib, Hasan
  • Is Part Of: Environmental epigenetics, 2023, Vol.9 (1), p.dvac029-dvac029
  • Description: Abstract Environmental effects on gene expression and offspring development can be mediated by epigenetic modifications. It is well established that maternal diet influences DNA methylation patterns and phenotypes in the offspring; however, the epigenetic effects of paternal diet on developing offspring warrants further investigation. Here, we examined how a prepubertal methionine-enriched paternal diet affected sperm DNA methylation and its subsequent effects on embryo gene expression. Three treatment and three control rams were bred to seven ewes, and blastocysts were flushed for RNA extraction. Semen was collected from all rams and submitted for reduced representation bisulfite sequencing analysis. In total, 166 differentially methylated cytosines were identified in the sperm from treatment versus control rams. Nine genes were found to be differentially expressed in embryos produced from treatment versus control rams, and seven differentially methylated cytosines in the sperm were found to be highly correlated with gene expression in the embryos. Our results demonstrate that sperm methylation differences induced by diet may influence fetal programming.
  • Publisher: UK: Oxford University Press
  • Language: English
  • Identifier: ISSN: 2058-5888
    EISSN: 2058-5888
    DOI: 10.1093/eep/dvac029
    PMID: 36727109
  • Source: PubMed Central
    Directory of Open Access Journals
    Open Access: Oxford University Press Open Journals
Back to results list
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait