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Preanalytical aspects and sample quality assessment in metabolomics studies of human blood

Clinical chemistry (Baltimore, Md.), 2013-05, Vol.59 (5), p.833-845 [Peer Reviewed Journal]

2013 American Association for Clinical Chemistry. ;COPYRIGHT 2013 American Association for Clinical Chemistry, Inc. ;Copyright American Association for Clinical Chemistry May 2013 ;ISSN: 0009-9147 ;EISSN: 1530-8561 ;DOI: 10.1373/clinchem.2012.199257 ;PMID: 23386698

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Title:
Preanalytical aspects and sample quality assessment in metabolomics studies of human blood
Author: Yin, Peiyuan ; Peter, Andreas ; Franken, Holger ; Zhao, Xinjie ; Neukamm, Sabine S ; Rosenbaum, Lars ; Lucio, Marianna ; Zell, Andreas ; Häring, Hans-Ulrich ; Xu, Guowang ; Lehmann, Rainer
Subjects: Blood ; Blood Chemical Analysis - methods ; Blood Chemical Analysis - standards ; Chromatography, Liquid ; Enzymes ; Hemolysis ; Humans ; Medical examination ; Medical research ; Medicine, Experimental ; Metabolic disorders ; Metabolites ; Metabolome ; Metabolomics ; Metabolomics - methods ; Metabolomics - standards ; Plasma ; Principal Component Analysis ; Quality Control ; Quality management ; Specimen Handling - methods ; Specimen Handling - standards ; Studies ; Tandem Mass Spectrometry
Is Part Of: Clinical chemistry (Baltimore, Md.), 2013-05, Vol.59 (5), p.833-845
Description: Metabolomics is a powerful tool that is increasingly used in clinical research. Although excellent sample quality is essential, it can easily be compromised by undetected preanalytical errors. We set out to identify critical preanalytical steps and biomarkers that reflect preanalytical inaccuracies. We systematically investigated the effects of preanalytical variables (blood collection tubes, hemolysis, temperature and time before further processing, and number of freeze-thaw cycles) on metabolomics studies of clinical blood and plasma samples using a nontargeted LC-MS approach. Serum and heparinate blood collection tubes led to chemical noise in the mass spectra. Distinct, significant changes of 64 features in the EDTA-plasma metabolome were detected when blood was exposed to room temperature for 2, 4, 8, and 24 h. The resulting pattern was characterized by increases in hypoxanthine and sphingosine 1-phosphate (800% and 380%, respectively, at 2 h). In contrast, the plasma metabolome was stable for up to 4 h when EDTA blood samples were immediately placed in iced water. Hemolysis also caused numerous changes in the metabolic profile. Unexpectedly, up to 4 freeze-thaw cycles only slightly changed the EDTA-plasma metabolome, but increased the individual variability. Nontargeted metabolomics investigations led to the following recommendations for the preanalytical phase: test the blood collection tubes, avoid hemolysis, place whole blood immediately in ice water, use EDTA plasma, and preferably use nonrefrozen biobank samples. To exclude outliers due to preanalytical errors, inspect the biomarker signal intensities reflecting systematic as well as accidental and preanalytical inaccuracies before processing the bioinformatics data.
Publisher: England: American Association for Clinical Chemistry, Inc
Language: English
Identifier: ISSN: 0009-9147
EISSN: 1530-8561
DOI: 10.1373/clinchem.2012.199257
PMID: 23386698
Source: AUTh Library subscriptions: ProQuest Central
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Preanalytical aspects and sample quality assessment in metabolomics studies of human blood

2013 American Association for Clinical Chemistry. ;COPYRIGHT 2013 American Association for Clinical Chemistry, Inc. ;Copyright American Association for Clinical Chemistry May 2013 ;ISSN: 0009-9147 ;EISSN: 1530-8561 ;DOI: 10.1373/clinchem.2012.199257 ;PMID: 23386698

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