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Beta cell differentiation during early human pancreas development

Journal of endocrinology, 2004-04, Vol.181 (1), p.11-23 [Peer Reviewed Journal]

ISSN: 0022-0795 ;EISSN: 1479-6805 ;DOI: 10.1677/joe.0.1810011 ;PMID: 15072563 ;CODEN: JOENAK

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Title:
Beta cell differentiation during early human pancreas development
Author: Piper, K ; Brickwood, S ; Turnpenny, LW ; Cameron, IT ; Ball, SG ; Wilson, DI ; Hanley, NA
Subjects: Animals ; Biological and medical sciences ; Biomarkers - analysis ; Cell Differentiation ; Cell Nucleus - chemistry ; Cells, Cultured ; Cytoplasm - chemistry ; Embryonic and Fetal Development - physiology ; Endocrine pancreas ; Epithelial Cells - chemistry ; Epithelial Cells - cytology ; Fundamental and applied biological sciences. Psychology ; Gestational Age ; Glucagon - analysis ; Homeodomain Proteins ; Humans ; Immunohistochemistry - methods ; Insulin - analysis ; Islets of Langerhans - chemistry ; Islets of Langerhans - cytology ; Islets of Langerhans - embryology ; Keratins - analysis ; Mice ; Morphology. Functional localizations ; Trans-Activators - analysis ; Vertebrates: endocrinology
Is Part Of: Journal of endocrinology, 2004-04, Vol.181 (1), p.11-23
Description: Understanding gene expression profiles during early human pancreas development is limited by comparison to studies in rodents. In this study, from the inception of pancreatic formation, embryonic pancreatic epithelial cells, approximately half of which were proliferative, expressed nuclear PDX1 and cytoplasmic CK19. Later, in the fetal pancreas, insulin was the most abundant hormone detected during the first trimester in largely non-proliferative cells. At sequential stages of early fetal development, as the number of insulin-positive cell clusters increased, the detection of CK19 in these cells diminished. PDX1 remained expressed in fetal beta cells. Vascular structures were present within the loose stroma surrounding pancreatic epithelial cells during embryogenesis. At 10 weeks post-conception (w.p.c.), all clusters containing more than ten insulin-positive cells had developed an intimate relationship with these vessels, compared with the remainder of the developing pancreas. At 12-13 w.p.c., human fetal islets, penetrated by vasculature, contained cells independently immunoreactive for insulin, glucagon, somatostatin and pancreatic polypeptide (PP), coincident with the expression of maturity markers prohormone convertase 1/3 (PC1/3), islet amyloid polypeptide, Chromogranin A and, more weakly, GLUT2. These data support the function of fetal beta cells as true endocrine cells by the end of the first trimester of human pregnancy.
Publisher: Colchester: BioScientifica
Language: English
Identifier: ISSN: 0022-0795
EISSN: 1479-6805
DOI: 10.1677/joe.0.1810011
PMID: 15072563
CODEN: JOENAK
Source: GFMER Free Medical Journals
MEDLINE

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Beta cell differentiation during early human pancreas development

ISSN: 0022-0795 ;EISSN: 1479-6805 ;DOI: 10.1677/joe.0.1810011 ;PMID: 15072563 ;CODEN: JOENAK

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