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Methodological Guidelines to Study Extracellular Vesicles

Circulation research, 2017-05, Vol.120 (10), p.1632-1648 [Peer Reviewed Journal]

2017 American Heart Association, Inc. ;Copyright Lippincott Williams & Wilkins Ovid Technologies May 12, 2017 ;ISSN: 0009-7330 ;ISSN: 1524-4571 ;EISSN: 1524-4571 ;DOI: 10.1161/CIRCRESAHA.117.309417 ;PMID: 28495994

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Title:
Methodological Guidelines to Study Extracellular Vesicles
Author: Coumans, Frank A.W ; Brisson, Alain R ; Buzas, Edit I ; Dignat-George, Françoise ; Drees, Esther E.E ; El-Andaloussi, Samir ; Emanueli, Costanza ; Gasecka, Aleksandra ; Hendrix, An ; Hill, Andrew F ; Lacroix, Romaric ; Lee, Yi ; van Leeuwen, Ton G ; Mackman, Nigel ; Mäger, Imre ; Nolan, John P ; van der Pol, Edwin ; Pegtel, D Michiel ; Sahoo, Susmita ; Siljander, Pia R.M ; Sturk, Guus ; de Wever, Olivier ; Nieuwland, Rienk
Subjects: Biomarkers - blood ; Blood Specimen Collection - methods ; Blood Specimen Collection - standards ; Body fluids ; Cardiovascular Diseases - blood ; Cardiovascular Diseases - diagnosis ; Exosomes - metabolism ; Extracellular Vesicles - metabolism ; Flow Cytometry - methods ; Humans ; Medicin och hälsovetenskap ; Vesicles
Is Part Of: Circulation research, 2017-05, Vol.120 (10), p.1632-1648
Description: Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.
Publisher: United States: American Heart Association, Inc
Language: English
Identifier: ISSN: 0009-7330
ISSN: 1524-4571
EISSN: 1524-4571
DOI: 10.1161/CIRCRESAHA.117.309417
PMID: 28495994
Source: GFMER Free Medical Journals
MEDLINE
SWEPUB Freely available online

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Methodological Guidelines to Study Extracellular Vesicles

2017 American Heart Association, Inc. ;Copyright Lippincott Williams & Wilkins Ovid Technologies May 12, 2017 ;ISSN: 0009-7330 ;ISSN: 1524-4571 ;EISSN: 1524-4571 ;DOI: 10.1161/CIRCRESAHA.117.309417 ;PMID: 28495994

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