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Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I

Proceedings of the National Academy of Sciences - PNAS, 2017-11, Vol.114 (48), p.12737-12742 [Peer Reviewed Journal]

Volumes 1–89 and 106–114, copyright as a collective work only; author(s) retains copyright to individual articles ;Copyright National Academy of Sciences Nov 28, 2017 ;2017 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.1714074114 ;PMID: 29133414

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Title:
Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I
Author: Fedor, Justin G. ; Jones, Andrew J. Y. ; Di Luca, Andrea ; Kaila, Ville R. I. ; Hirst, Judy
Subjects: Adenosine triphosphate ; Amino Acid Motifs ; Animals ; Binding Sites ; Biocatalysis ; Biological Sciences ; Catalysis ; Cattle ; Cells ; Coenzyme Q10 ; Correlation analysis ; Electron transfer ; Electron transport chain ; Electron Transport Complex I - chemistry ; Electron Transport Complex I - genetics ; Electron Transport Complex I - metabolism ; Energy ; Energy consumption ; Energy conversion ; Energy of dissociation ; Gene Expression ; Hydrophobic and Hydrophilic Interactions ; Hydrophobicity ; Hydroquinone ; Kinetics ; Mammalian cells ; Mitochondria ; Mitochondria, Heart - chemistry ; Mitochondria, Heart - enzymology ; Models, Molecular ; NADH ; NADH-ubiquinone oxidoreductase ; Nicotinamide adenine dinucleotide ; Oxidoreductases - chemistry ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Structure, Secondary ; Proteolipids - chemistry ; Proteolipids - metabolism ; Protons ; Quinol oxidase ; Quinones ; Reaction kinetics ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Static Electricity ; Substrate Specificity ; Swine ; Terpenes ; Terpenes - chemistry ; Terpenes - metabolism ; Thermodynamics ; Thermus thermophilus - chemistry ; Thermus thermophilus - enzymology ; Translocation ; Travel time ; Ubiquinol ; Ubiquinone ; Ubiquinone - analogs & derivatives ; Ubiquinone - chemistry ; Ubiquinone - metabolism ; Ubiquinone oxidoreductase
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2017-11, Vol.114 (48), p.12737-12742
Description: Respiratory complex I (NADH:ubiquinone oxidoreductase), one of the largest membrane-bound enzymes in mammalian cells, powers ATP synthesis by using the energy from electron transfer from NADH to ubiquinone-10 to drive protons across the energy-transducing mitochondrial inner membrane. Ubiquinone-10 is extremely hydrophobic, but in complex I the binding site for its redox-active quinone headgroup is ∼20 Å above the membrane surface. Structural data suggest it accesses the site by a narrow channel, long enough to accommodate almost all of its ∼50-Å isoprenoid chain. However, how ubiquinone/ubiquinol exchange occurs on catalytically relevant timescales, and whether binding/dissociation events are involved in coupling electron transfer to proton translocation, are unknown. Here, we use proteoliposomes containing complex I, together with a quinol oxidase, to determine the kinetics of complex I catalysis with ubiquinones of varying isoprenoid chain length, from 1 to 10 units. We interpret our results using structural data, which show the hydrophobic channel is interrupted by a highly charged region at isoprenoids 4–7. We demonstrate that ubiquinol-10 dissociation is not rate determining and deduce that ubiquinone-10 has both the highest binding affinity and the fastest binding rate. We propose that the charged region and chain directionality assist product dissociation, and that isoprenoid stepping ensures short transit times. These properties of the channel do not benefit the exhange of short-chain quinones, for which product dissociation may become rate limiting. Thus, we discuss how the long channel does not hinder catalysis under physiological conditions and the possible roles of ubiquinone/ubiquinol binding/dissociation in energy conversion.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.1714074114
PMID: 29133414
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central

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Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I

Volumes 1–89 and 106–114, copyright as a collective work only; author(s) retains copyright to individual articles ;Copyright National Academy of Sciences Nov 28, 2017 ;2017 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.1714074114 ;PMID: 29133414

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