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Elevated levels of circulating DNA in cardiovascular disease patients: metagenomic profiling of microbiome in the circulation

PloS one, 2014-08, Vol.9 (8), p.e105221-e105221 [Peer Reviewed Journal]

COPYRIGHT 2014 Public Library of Science ;COPYRIGHT 2014 Public Library of Science ;2014 Dinakaran et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2014 Dinakaran et al 2014 Dinakaran et al ;ISSN: 1932-6203 ;EISSN: 1932-6203 ;DOI: 10.1371/journal.pone.0105221 ;PMID: 25133738

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Title:
Elevated levels of circulating DNA in cardiovascular disease patients: metagenomic profiling of microbiome in the circulation
Author: Dinakaran, Vasudevan ; Rathinavel, Andiappan ; Pushpanathan, Muthuirulan ; Sivakumar, Ramamoorthy ; Gunasekaran, Paramasamy ; Rajendhran, Jeyaprakash
Bereswill, Stefan
Subjects: Acquired immune deficiency syndrome ; Actinobacteria - genetics ; Adolescent ; Adult ; AIDS ; Analysis ; Bacteria ; beta-Globins - genetics ; Biology and Life Sciences ; Cardiac patients ; Cardiovascular disease ; Cardiovascular diseases ; Cardiovascular Diseases - blood ; Cardiovascular Diseases - microbiology ; Cardiovascular Diseases - virology ; Deoxyribonucleic acid ; DNA ; DNA - blood ; DNA, Bacterial - blood ; DNA, Bacterial - genetics ; DNA, Viral - blood ; DNA, Viral - genetics ; Female ; Heart attacks ; HIV ; Human immunodeficiency virus ; Humans ; Infections ; Ischemia ; Lymphocystis ; Male ; Medicine and Health Sciences ; Metagenomics ; Microbiomes ; Microorganisms ; Middle Aged ; Patients ; Phages ; Plasma ; Propionibacterium - genetics ; Proteobacteria - genetics ; Pseudomonas Phages - genetics ; RNA, Ribosomal, 16S - blood ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Taxonomy ; Viruses ; Young Adult
Is Part Of: PloS one, 2014-08, Vol.9 (8), p.e105221-e105221
Description: Cardiovascular diseases (CVDs) are the leading cause of death worldwide. An expanding body of evidence supports the role of human microbiome in the establishment of CVDs and, this has gained much attention recently. This work was aimed to study the circulating human microbiome in CVD patients and healthy subjects. The levels of circulating cell free DNA (circDNA) was higher in CVD patients (n = 80) than in healthy controls (n = 40). More specifically, the relative levels of circulating bacterial DNA and the ratio of 16S rRNA/β-globin gene copy numbers were higher in the circulation of CVD patients than healthy individuals. In addition, we found a higher circulating microbial diversity in CVD patients (n = 3) in comparison to healthy individuals (n = 3) by deep shotgun sequencing. At the phylum level, we observed a dominance of Actinobacteria in CVD patients, followed by Proteobacteria, in contrast to that in healthy controls, where Proteobacteria was predominantly enriched, followed by Actinobacteria. The circulating virome in CVD patients was enriched with bacteriophages with a preponderance of Propionibacterium phages, followed by Pseudomonas phages and Rhizobium phages in contrast to that in healthy individuals, where a relatively greater abundance of eukaryotic viruses dominated by Lymphocystis virus (LCV) and Torque Teno viruses (TTV) was observed. Thus, the release of bacterial and viral DNA elements in the circulation could play a major role leading to elevated circDNA levels in CVD patients. The increased circDNA levels could be either the cause or consequence of CVD incidence, which needs to be explored further.
Publisher: United States: Public Library of Science
Language: English
Identifier: ISSN: 1932-6203
EISSN: 1932-6203
DOI: 10.1371/journal.pone.0105221
PMID: 25133738
Source: Freely Accessible Journals
PLoS
MEDLINE
PubMed Central
ProQuest Central
DOAJ Directory of Open Access Journals

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Elevated levels of circulating DNA in cardiovascular disease patients: metagenomic profiling of microbiome in the circulation

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