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Role of NADPH Oxidase in Total Salvianolic Acid Injection Attenuating Ischemia-Reperfusion Impaired Cerebral Microcirculation and Neurons: Implication of AMPK/Akt/PKC

Microcirculation (New York, N.Y. 1994), 2014-10, Vol.21 (7), p.615-627 [Peer Reviewed Journal]

2014 John Wiley & Sons Ltd ;2014 John Wiley & Sons Ltd. ;Copyright © 2014 John Wiley & Sons Ltd ;ISSN: 1073-9688 ;EISSN: 1549-8719 ;DOI: 10.1111/micc.12140 ;PMID: 24702968

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Title:
Role of NADPH Oxidase in Total Salvianolic Acid Injection Attenuating Ischemia-Reperfusion Impaired Cerebral Microcirculation and Neurons: Implication of AMPK/Akt/PKC
Author: Tang, Hao ; Pan, Chun-Shui ; Mao, Xiao-Wei ; Liu, Yu-Ying ; Yan, Li ; Zhou, Chang-Man ; Fan, Jing-Yu ; Zhang, Shuang-Yan ; Han, Jing-Yan
Subjects: Alkenes - pharmacology ; Alkenes - therapeutic use ; AMP-Activated Protein Kinases - physiology ; AMPK/Akt/PKC ; Animals ; antiapoptosis ; antioxidation ; Apoptosis - drug effects ; Capillary Permeability - drug effects ; Cerebral Infarction - etiology ; Cerebral Infarction - pathology ; Cerebral Infarction - prevention & control ; Drugs, Chinese Herbal - pharmacology ; Drugs, Chinese Herbal - therapeutic use ; Infarction, Middle Cerebral Artery - drug therapy ; Infarction, Middle Cerebral Artery - enzymology ; Infarction, Middle Cerebral Artery - physiopathology ; Leukocytes - drug effects ; Lipid Peroxidation - drug effects ; Male ; Microcirculation - drug effects ; microvasculature ; Movement Disorders - etiology ; Movement Disorders - prevention & control ; NADPH ; NADPH Oxidases - physiology ; Nerve Tissue Proteins - physiology ; Neurons - drug effects ; Neurons - enzymology ; Phosphorylation - drug effects ; Polyphenols - pharmacology ; Polyphenols - therapeutic use ; Protein Kinase C - physiology ; Protein Transport - drug effects ; Proto-Oncogene Proteins c-akt - physiology ; Rats ; Rats, Sprague-Dawley ; Reactive Oxygen Species - metabolism ; Reperfusion Injury - drug therapy ; Reperfusion Injury - enzymology ; Reperfusion Injury - physiopathology ; Signal Transduction - drug effects
Is Part Of: Microcirculation (New York, N.Y. 1994), 2014-10, Vol.21 (7), p.615-627
Description: Objective TSI is a new drug derived from Chinese medicine for treatment of ischemic stroke in China. The aim of this study was to verify the therapeutic effect of TSI in a rat model of MCAO, and further explore the mechanism for its effect. Methods Male Sprague–Dawley rats were subjected to right MCAO for 60 minutes followed by reperfusion. TSI (1.67 mg/kg) was administrated before reperfusion via femoral vein injection. Twenty‐four hours after reperfusion, the fluorescence intensity of DHR 123 in, leukocyte adhesion to and albumin leakage from the cerebral venules were observed. Neurological scores, TTC staining, brain water content, Nissl staining, TUNEL staining, and MDA content were assessed. Bcl‐2/Bax, cleaved caspase‐3, NADPH oxidase subunits p47phox/p67phox/gp91phox, and AMPK/Akt/PKC were analyzed by Western blot. Results TSI attenuated I/R‐induced microcirculatory disturbance and neuron damage, activated AMPK, inhibited NADPH oxidase subunits membrane translocation, suppressed Akt phosphorylation, and PKC translocation. Conclusions TSI attenuates I/R‐induced brain injury in rats, supporting its clinic use for treatment of acute ischemic stroke. The role of TSI may benefit from its antioxidant activity, which is most likely implemented via inactivation of NADPH oxidase through a signaling pathway implicating AMPK/Akt/PKC.
Publisher: United States: Blackwell Publishing Ltd
Language: English
Identifier: ISSN: 1073-9688
EISSN: 1549-8719
DOI: 10.1111/micc.12140
PMID: 24702968
Source: MEDLINE
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Role of NADPH Oxidase in Total Salvianolic Acid Injection Attenuating Ischemia-Reperfusion Impaired Cerebral Microcirculation and Neurons: Implication of AMPK/Akt/PKC

2014 John Wiley & Sons Ltd ;2014 John Wiley & Sons Ltd. ;Copyright © 2014 John Wiley & Sons Ltd ;ISSN: 1073-9688 ;EISSN: 1549-8719 ;DOI: 10.1111/micc.12140 ;PMID: 24702968

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