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High-throughput sequencing of amplicons for monitoring yeast biodiversity in must and during alcoholic fermentation

Journal of industrial microbiology & biotechnology, 2014-05, Vol.41 (5), p.811-821 [Peer Reviewed Journal]

Society for Industrial Microbiology and Biotechnology 2014 ;2015 INIST-CNRS ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 1367-5435 ;EISSN: 1476-5535 ;DOI: 10.1007/s10295-014-1427-2 ;PMID: 24659178

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Title:
High-throughput sequencing of amplicons for monitoring yeast biodiversity in must and during alcoholic fermentation
Author: David, Vanessa ; Terrat, Sébastien ; Herzine, Khaled ; Claisse, Olivier ; Rousseaux, Sandrine ; Tourdot-Maréchal, Raphaëlle ; Masneuf-Pomarede, Isabelle ; Ranjard, Lionel ; Alexandre, Hervé
Subjects: alcoholic fermentation ; Analysis ; Biochemistry ; Biodiversity ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Chemical and Process Engineering ; Denaturing Gradient Gel Electrophoresis ; Engineering Sciences ; Environmental Microbiology ; Environmental Sciences ; Ethanol - metabolism ; Fermentation ; Food engineering ; Fundamental and applied biological sciences. Psychology ; Genetic Engineering ; grapes ; High-Throughput Nucleotide Sequencing ; Inorganic Chemistry ; Life Sciences ; Microbiology ; monitoring ; Polymerase Chain Reaction ; Population ; population dynamics ; sequence analysis ; Studies ; Vegetal Biology ; Vitaceae ; Vitis - microbiology ; Wineries & vineyards ; Wines ; Yeast ; yeasts ; Yeasts - classification ; Yeasts - genetics
Is Part Of: Journal of industrial microbiology & biotechnology, 2014-05, Vol.41 (5), p.811-821
Description: We compared pyrosequencing technology with the PCR-ITS-RFLP analysis of yeast isolates and denaturing gradient gel electrophoresis (DGGE). These methods gave divergent findings for the yeast population. DGGE was unsuitable for the quantification of biodiversity and its use for species detection was limited by the initial abundance of each species. The isolates identified by PCR-ITS-RFLP were not fully representative of the true population. For population dynamics, high-throughput sequencing technology yielded results differing in some respects from those obtained with other approaches. This study demonstrates that 454 pyrosequencing of amplicons is more relevant than other methods for studying the yeast community on grapes and during alcoholic fermentation. Indeed, this high-throughput sequencing method detected larger numbers of species on grapes and identified species present during alcoholic fermentation that were undetectable with the other techniques.
Publisher: Berlin/Heidelberg: Springer-Verlag
Language: English
Identifier: ISSN: 1367-5435
EISSN: 1476-5535
DOI: 10.1007/s10295-014-1427-2
PMID: 24659178
Source: MEDLINE
Alma/SFX Local Collection
ProQuest Central

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High-throughput sequencing of amplicons for monitoring yeast biodiversity in must and during alcoholic fermentation

Society for Industrial Microbiology and Biotechnology 2014 ;2015 INIST-CNRS ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 1367-5435 ;EISSN: 1476-5535 ;DOI: 10.1007/s10295-014-1427-2 ;PMID: 24659178

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