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Hypertonic saline alleviates experimentally induced cerebral oedema through suppression of vascular endothelial growth factor and its receptor VEGFR2 expression in astrocytes

BMC neuroscience, 2016-10, Vol.17 (1), p.64-64, Article 64 [Peer Reviewed Journal]

COPYRIGHT 2016 BioMed Central Ltd. ;Copyright BioMed Central 2016 ;The Author(s) 2016 ;ISSN: 1471-2202 ;EISSN: 1471-2202 ;DOI: 10.1186/s12868-016-0299-y ;PMID: 27733124

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Title:
Hypertonic saline alleviates experimentally induced cerebral oedema through suppression of vascular endothelial growth factor and its receptor VEGFR2 expression in astrocytes
Author: Huang, Linqiang ; Cao, Wei ; Deng, Yiyu ; Zhu, Gaofeng ; Han, Yongli ; Zeng, Hongke
Subjects: Animals ; Astrocytes ; Astrocytes - drug effects ; Astrocytes - metabolism ; Blood-Brain Barrier - drug effects ; Blood-Brain Barrier - metabolism ; Blood-Brain Barrier - pathology ; Brain Edema - drug therapy ; Brain Edema - metabolism ; Brain Edema - pathology ; Capillary Permeability - drug effects ; Capillary Permeability - physiology ; Care and treatment ; Cell Hypoxia - drug effects ; Cell Hypoxia - physiology ; Cells, Cultured ; Claudin-5 - metabolism ; Disease Models, Animal ; Dosage and administration ; Dropsy ; Edema ; Glucose - deficiency ; Infarction, Middle Cerebral Artery ; Male ; Neuroprotective Agents - pharmacology ; Physiologic salines ; Random Allocation ; Rats, Sprague-Dawley ; Reperfusion Injury - drug therapy ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; RNA ; RNA, Messenger - metabolism ; Saline Solution, Hypertonic - pharmacology ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism ; Vascular Endothelial Growth Factor Receptor-2 - metabolism ; Zonula Occludens-1 Protein - metabolism
Is Part Of: BMC neuroscience, 2016-10, Vol.17 (1), p.64-64, Article 64
Description: Cerebral oedema is closely related to the permeability of blood-brain barrier, vascular endothelial growth factor (VEGF) and its receptor vascular endothelial growth factor receptor 2 (VEGFR2) all of which are important blood-brain barrier (BBB) permeability regulatory factors. Zonula occludens 1 (ZO-1) and claudin-5 are also the key components of BBB. Hypertonic saline is widely used to alleviate cerebral oedema. This study aimed to explore the possible mechanisms underlying hypertonic saline that ameliorates cerebral oedema effectively. Middle cerebral artery occlusion (MCAO) model in Sprague-Dawley (SD) rats and of oxygen-glucose deprivation model in primary astrocytes were used in this study. The brain water content (BWC) was used to assess the effect of 10 % HS on cerebral oedema. The assessment of Evans blue (EB) extravasation was performed to evaluate the protective effect of 10 % HS on blood-brain barrier. The quantification of VEGF, VEGFR2, ZO-1 and claudin-5 was used to illustrate the mechanism of 10 % HS ameliorating cerebral oedema. BWC was analysed by wet-to-dry ratios in the ischemic hemisphere of SD rats; it was significantly decreased after 10 % HS treatment (P < 0.05). We also investigated the blood-brain barrier protective effect by 10 % HS which reduced EB extravasation effectively in the peri-ischemic brain tissue. In parallel to the above notably at 24 h following MCAO, mRNA and protein expression of VEGF and VEGFR2 in the peri-ischemic brain tissue was down-regulated after 10 % HS treatment (P < 0.05). Along with this, in vitro studies showed increased VEGF and VEGFR2 mRNA and protein expression in primary astrocytes under hypoxic condition (P < 0.05), but it was suppressed after HS treatment (P < 0.05). In addition, HS inhibited the down-regulation of ZO-1, claudin-5 effectively. The results suggest that 10 % HS could alleviate cerebral oedema possibly through reducing the ischemia induced BBB permeability as a consequence of inhibiting VEGF-VEGFR2-mediated down-regulation of ZO-1, claudin-5.
Publisher: England: BioMed Central Ltd
Language: English
Identifier: ISSN: 1471-2202
EISSN: 1471-2202
DOI: 10.1186/s12868-016-0299-y
PMID: 27733124
Source: ProQuest One Psychology
GFMER Free Medical Journals
MEDLINE
PubMed Central
ROAD: Directory of Open Access Scholarly Resources
ProQuest Central
DOAJ Directory of Open Access Journals
Springer Nature OA Free Journals

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Hypertonic saline alleviates experimentally induced cerebral oedema through suppression of vascular endothelial growth factor and its receptor VEGFR2 expression in astrocytes

COPYRIGHT 2016 BioMed Central Ltd. ;Copyright BioMed Central 2016 ;The Author(s) 2016 ;ISSN: 1471-2202 ;EISSN: 1471-2202 ;DOI: 10.1186/s12868-016-0299-y ;PMID: 27733124

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