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Optimizing Hexose Utilization Pathways of ICupriavidus necator/I for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

International journal of molecular sciences, 2023-12, Vol.25 (1) [Peer Reviewed Journal]

COPYRIGHT 2023 MDPI AG ;ISSN: 1422-0067 ;EISSN: 1422-0067 ;DOI: 10.3390/ijms25010548

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  • Title:
    Optimizing Hexose Utilization Pathways of ICupriavidus necator/I for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions
  • Author: Wang, Lei ; Luo, Huiying ; Yao, Bin ; Yao, Junhu ; Zhang, Jie
  • Subjects: Enzymes ; Physiological aspects
  • Is Part Of: International journal of molecular sciences, 2023-12, Vol.25 (1)
  • Description: Cupriavidus necator is a versatile microbial chassis to produce high-value products. Blocking the poly-β-hydroxybutyrate synthesis pathway (encoded by the phaC1AB1 operon) can effectively enhance the production of C. necator, but usually decreases cell density in the stationary phase. To address this problem, we modified the hexose utilization pathways of C. necator in this study by implementing strategies such as blocking the Entner–Doudoroff pathway, completing the phosphopentose pathway by expressing the gnd gene (encoding 6-phosphogluconate dehydrogenase), and completing the Embden–Meyerhof–Parnas pathway by expressing the pfkA gene (encoding 6-phosphofructokinase). During heterotrophic fermentation, the OD[sub.600] of the phaC1AB1-knockout strain increased by 44.8% with pfkA gene expression alone, and by 93.1% with gnd and pfkA genes expressing simultaneously. During autotrophic fermentation, gnd and pfkA genes raised the OD[sub.600] of phaC1AB1-knockout strains by 19.4% and 12.0%, respectively. To explore the effect of the pfkA gene on the production of C. necator, an alanine-producing C. necator was constructed by expressing the NADPH-dependent L-alanine dehydrogenase, alanine exporter, and knocking out the phaC1AB1 operon. The alanine-producing strain had maximum alanine titer and yield of 784 mg/L and 11.0%, respectively. And these values were significantly improved to 998 mg/L and 13.4% by expressing the pfkA gene. The results indicate that completing the Embden–Meyerhof–Parnas pathway by expressing the pfkA gene is an effective method to improve the growth and production of C. necator.
  • Publisher: MDPI AG
  • Language: English
  • Identifier: ISSN: 1422-0067
    EISSN: 1422-0067
    DOI: 10.3390/ijms25010548
  • Source: Open Access: DOAJ Directory of Open Access Journals
    Open Access: PubMed Central
    AUTh Library subscriptions: ProQuest Central
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