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Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions

FEBS letters, 2002-10, Vol.530 (1), p.24-30 [Peer Reviewed Journal]

2002 Federation of European Biochemical Societies ;ISSN: 0014-5793 ;EISSN: 1873-3468 ;DOI: 10.1016/S0014-5793(02)03346-X ;PMID: 12387860

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Title:
Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions
Author: Evans, Lewis D.B ; Roszak, Aleksander W ; Noble, Lorna J ; Robinson, David A ; Chalk, Peter A ; Matthews, Jennifer L ; Coggins, John R ; Price, Nicholas C ; Lapthorn, Adrian J
Subjects: 3-Dehydroquinic acid ; Crystallography, X-Ray ; Dehydroquinase ; Hydro-Lyases - chemistry ; Hydro-Lyases - metabolism ; Models, Molecular ; Mycobacterium tuberculosis - enzymology ; Nuclear Magnetic Resonance, Biomolecular ; Phosphate ; Polymers - metabolism ; Protein Binding ; Protein Conformation ; Streptomyces - enzymology ; Substrate recognition ; Substrate Specificity ; Sulfate ; X-ray crystallography
Is Part Of: FEBS letters, 2002-10, Vol.530 (1), p.24-30
Description: The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the case of the latter enzyme. The data provide new insights into the differences between the two enzymes in terms of substrate recognition and catalytic efficiency and may also explain the relative potencies of rationally designed inhibitors. An improved route to the synthesis of the substrate 3-dehydroquinic acid (dehydroquinate) is described.
Publisher: England: Elsevier B.V
Language: English
Identifier: ISSN: 0014-5793
EISSN: 1873-3468
DOI: 10.1016/S0014-5793(02)03346-X
PMID: 12387860
Source: Geneva Foundation Free Medical Journals at publisher websites
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Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions

2002 Federation of European Biochemical Societies ;ISSN: 0014-5793 ;EISSN: 1873-3468 ;DOI: 10.1016/S0014-5793(02)03346-X ;PMID: 12387860

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